The preterm pig as a model of premature infant gait ataxia

A. Bergström, K. Ryom, C. Vanden Hole, A. D. Andersen, Kerstin Skovgaard, C. Van Ginneken, T. Thymann, P. T. Sangild

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    Abstract

    Aims/background Compromised gait, balance and motor coordination (ataxia) as observed in cases of cerebral palsy is a serious complication to premature birth. The cerebellum is a central region with regards to these brain functions and its development shows high sensitivity to premature birth. Our group has over many years refined a pig model of premature birth focusing on gut and immune system development. Phenotypically, we have observed distinct motoric problems e.g. falls, tiptoe walking and swaying in preterm pigs relative to term born counterparts, indicating compromised brain function. The aim of this study was to compare gait patterns and cerebellar neurodevelopmental gene expression of preterm and term piglets. Methods We compared gait patterns and T-maze performance of caesarean born preterm (3 litters, 90% gestation) and term born pigs (1 litter, 100% gestation) recorded at five distinct postnatal days. MatLab was used to determine a list of spatiotemporal gait characteristics e.g. stride length/ frequency, ”duty factor” and asymmetry indices. These data were paralleled by qPCR of >60 selected neurodevelopmental genes of isolated cerebellar tissue. Results While most genes did not differ significantly, we found higher (fold change [1,5-2]) mRNA levels of Midkine, Doublecortin, Neurotrophin3, p75 and Ephrin-B1 in preterms. Preliminary results from gait and T-maze showed significant functional differences between terms and preterms. Conclusions The preterm pig shows functional delays relative to terms, yet the limited cerebellar gene expression differences (mainly related to angiogenesis) suggest other brain regions e.g. motor cortex and basal ganglia to also be involved in compromised gait.
    Original languageEnglish
    Publication date2016
    Number of pages1
    Publication statusPublished - 2016
    Event10th FENS Forum of Neuroscience 2016 - Copenhagen, Denmark
    Duration: 2 Jul 20166 Jul 2016
    Conference number: 10
    http://forum2016.fens.org/

    Conference

    Conference10th FENS Forum of Neuroscience 2016
    Number10
    CountryDenmark
    CityCopenhagen
    Period02/07/201606/07/2016
    Internet address

    Cite this

    Bergström, A., Ryom, K., Vanden Hole, C., Andersen, A. D., Skovgaard, K., Van Ginneken, C., Thymann, T., & Sangild, P. T. (2016). The preterm pig as a model of premature infant gait ataxia. Abstract from 10th FENS Forum of Neuroscience 2016, Copenhagen, Denmark.