The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperione-usher pathway system assembling fimbriae

Caroline Giraud, Christophe S. Bernard, Virginie Calderon, Liang Yang, Alain Filloux, Søren Molin, Gwennaele Fichant, Christophe Bordi, Sophie de Bentzmann

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The opportunistic pathogen Pseudomonas aeruginosa has redundant molecular systems that contribute to its pathogenicity. Those assembling fimbrial structures promote complex organized community lifestyle. We characterized a new 5.8 kb genetic locus, cupE, that includes the conserved usher- and chaperone-encoding genes. This locus, widely conserved in different bacterial species, contains four additional genes encoding non-archetypal fimbrial subunits. We first evidenced that the cupE gene cluster was specifically expressed in biofilm conditions and was responsible for fibre assembly containing at least CupE1 protein, at the bacterial cell surface. These fimbriae not only played a significant role in the early stages (microcolony and macrocolony formation) but also in shaping 3D mushrooms during P. aeruginosa biofilm development. Using wide-genome transposon mutagenesis, we identified the PprAB two-component system (TCS) as a regulator of cupE expression, and further demonstrated the involvement of the PprAB TCS in direct CupE fimbrial assembly activation. Thus, this TCS represents a new regulatory element controlling the transition between planktonic and community lifestyles in P. aeruginosa.
    Original languageEnglish
    JournalEnvironmental Microbiology
    Volume13
    Issue number3
    Pages (from-to)666-683
    ISSN1462-2912
    DOIs
    Publication statusPublished - 2011

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