The plasma membrane proteome of germinating barley embryos

Radovan Hynek, Birte Svensson, O.N. Jensen, Vibeke Barkholt, Christine Finnie

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Cereal seed germination involves a complex coordination between different seed tissues. Plasma membranes must play crucial roles in coordination and execution of germination; however, very little is known about seed plasma membrane proteomes due to limited tissue amounts combined with amphiphilicity and low abundance of membrane proteins. A fraction enriched in plasma membranes was prepared from embryos dissected from 18 h germinated barley seeds using aqueous two-phase partitioning. Reversed-phase chromatography on C-4 resin performed in micro-spin columns with stepwise elution by 2-propanol was used to reduce soluble protein contamination and enrich for hydrophobic proteins. Sixty-one proteins in 14 SDS-PAGE bands were identified by LC-MS/MS and database searches. The identifications provide new insight into the plasma membrane functions in seed germination.
    Original languageEnglish
    JournalProteomics
    Volume9
    Issue number14
    Pages (from-to)3787-3794
    ISSN1615-9853
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Reversed-phase LC
    • Cereal seed germination
    • Barley embryo
    • Plasma membrane proteins

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