The pig as a large animal model for characterization of host-pathogen interactions

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Abstract

Large animal models are essential in understanding the mechanisms involved in human infectious disease. To study the expression of host and bacterial genes involved in defense and survival mechanisms, we analyzed lung tissue from pigs experimentally infected with the Gram-negative bacterium A. pleuropneumoniae. All steps including RNA extraction and high-throughput real-time qPCR were carried out simultaneously for the two organisms. By applying this dual-organism approach, we obtained unique insights into the host-pathogen interaction at the site of infection. Differential expression of host genes involved in innate immune responses towards Gram-negative infections, including pattern recognition receptors and cytokines concurrent with expression of bacterial genes involved in lipopolysaccharide biosynthesis and adhesion was demonstrated.

We also studied the gene expression in blood leukocytes after experimental H1N2 virus infection of pigs, and found the regulation of several swine encoded miRNAs and cytokines to mimic key findings from influenza studies in human patients. By employing the pig as a model we were able to perform highly controlled experimental infections and to study changes of symptoms, viral titer, and expression of microRNAs/mRNAs as the influenza infection progressed in time, generating information that would be difficult to obtain from human patients.
Original languageEnglish
Publication date2014
Publication statusPublished - 2014
EventImmunology and Infectious Diseases - Sandbjderg Gods, Sandbjderg , Denmark
Duration: 3 Sep 20145 Sep 2014

Conference

ConferenceImmunology and Infectious Diseases
LocationSandbjderg Gods
CountryDenmark
CitySandbjderg
Period03/09/201405/09/2014

Cite this

Skovgaard, K., Brogaard, L., Heegaard, P. M. H., & Schou, K. K. (2014). The pig as a large animal model for characterization of host-pathogen interactions. Abstract from Immunology and Infectious Diseases, Sandbjderg , Denmark.
Skovgaard, Kerstin ; Brogaard, Louise ; Heegaard, Peter M. H. ; Schou, Kirstine Klitgaard. / The pig as a large animal model for characterization of host-pathogen interactions. Abstract from Immunology and Infectious Diseases, Sandbjderg , Denmark.
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title = "The pig as a large animal model for characterization of host-pathogen interactions",
abstract = "Large animal models are essential in understanding the mechanisms involved in human infectious disease. To study the expression of host and bacterial genes involved in defense and survival mechanisms, we analyzed lung tissue from pigs experimentally infected with the Gram-negative bacterium A. pleuropneumoniae. All steps including RNA extraction and high-throughput real-time qPCR were carried out simultaneously for the two organisms. By applying this dual-organism approach, we obtained unique insights into the host-pathogen interaction at the site of infection. Differential expression of host genes involved in innate immune responses towards Gram-negative infections, including pattern recognition receptors and cytokines concurrent with expression of bacterial genes involved in lipopolysaccharide biosynthesis and adhesion was demonstrated. We also studied the gene expression in blood leukocytes after experimental H1N2 virus infection of pigs, and found the regulation of several swine encoded miRNAs and cytokines to mimic key findings from influenza studies in human patients. By employing the pig as a model we were able to perform highly controlled experimental infections and to study changes of symptoms, viral titer, and expression of microRNAs/mRNAs as the influenza infection progressed in time, generating information that would be difficult to obtain from human patients.",
author = "Kerstin Skovgaard and Louise Brogaard and Heegaard, {Peter M. H.} and Schou, {Kirstine Klitgaard}",
year = "2014",
language = "English",
note = "Immunology and Infectious Diseases ; Conference date: 03-09-2014 Through 05-09-2014",

}

Skovgaard, K, Brogaard, L, Heegaard, PMH & Schou, KK 2014, 'The pig as a large animal model for characterization of host-pathogen interactions' Immunology and Infectious Diseases, Sandbjderg , Denmark, 03/09/2014 - 05/09/2014, .

The pig as a large animal model for characterization of host-pathogen interactions. / Skovgaard, Kerstin; Brogaard, Louise; Heegaard, Peter M. H.; Schou, Kirstine Klitgaard.

2014. Abstract from Immunology and Infectious Diseases, Sandbjderg , Denmark.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - The pig as a large animal model for characterization of host-pathogen interactions

AU - Skovgaard, Kerstin

AU - Brogaard, Louise

AU - Heegaard, Peter M. H.

AU - Schou, Kirstine Klitgaard

PY - 2014

Y1 - 2014

N2 - Large animal models are essential in understanding the mechanisms involved in human infectious disease. To study the expression of host and bacterial genes involved in defense and survival mechanisms, we analyzed lung tissue from pigs experimentally infected with the Gram-negative bacterium A. pleuropneumoniae. All steps including RNA extraction and high-throughput real-time qPCR were carried out simultaneously for the two organisms. By applying this dual-organism approach, we obtained unique insights into the host-pathogen interaction at the site of infection. Differential expression of host genes involved in innate immune responses towards Gram-negative infections, including pattern recognition receptors and cytokines concurrent with expression of bacterial genes involved in lipopolysaccharide biosynthesis and adhesion was demonstrated. We also studied the gene expression in blood leukocytes after experimental H1N2 virus infection of pigs, and found the regulation of several swine encoded miRNAs and cytokines to mimic key findings from influenza studies in human patients. By employing the pig as a model we were able to perform highly controlled experimental infections and to study changes of symptoms, viral titer, and expression of microRNAs/mRNAs as the influenza infection progressed in time, generating information that would be difficult to obtain from human patients.

AB - Large animal models are essential in understanding the mechanisms involved in human infectious disease. To study the expression of host and bacterial genes involved in defense and survival mechanisms, we analyzed lung tissue from pigs experimentally infected with the Gram-negative bacterium A. pleuropneumoniae. All steps including RNA extraction and high-throughput real-time qPCR were carried out simultaneously for the two organisms. By applying this dual-organism approach, we obtained unique insights into the host-pathogen interaction at the site of infection. Differential expression of host genes involved in innate immune responses towards Gram-negative infections, including pattern recognition receptors and cytokines concurrent with expression of bacterial genes involved in lipopolysaccharide biosynthesis and adhesion was demonstrated. We also studied the gene expression in blood leukocytes after experimental H1N2 virus infection of pigs, and found the regulation of several swine encoded miRNAs and cytokines to mimic key findings from influenza studies in human patients. By employing the pig as a model we were able to perform highly controlled experimental infections and to study changes of symptoms, viral titer, and expression of microRNAs/mRNAs as the influenza infection progressed in time, generating information that would be difficult to obtain from human patients.

M3 - Conference abstract for conference

ER -

Skovgaard K, Brogaard L, Heegaard PMH, Schou KK. The pig as a large animal model for characterization of host-pathogen interactions. 2014. Abstract from Immunology and Infectious Diseases, Sandbjderg , Denmark.