The occurrence of mycoplasmas in the lungs of swine in Gran Canaria (Spain)

P. Assuncao, C. De la Fe, Branko Kokotovic, O. Gonzalez, J.B. Poveda

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    Abstract

    The study was conducted to investigate the mycoplasmal flora in the lungs of pigs with enzootic pneumonia at Gran Canaria (Spain). From 54 pneumonic lungs collected at an abattoir, 85 isolates were cultivated. On the basis of cultural and biochemical characteristics, the isolates were preliminarily identified as Mycoplasma species. Using different species-specific PCRs, 40, 27, 11 and 7 of the isolates were identified as M hyorhinis, M. hyopneumoniae, M. hyosynoviae and M. flocculare, respectively. Nine of the M. hyopneumoniae cultures were found to be in mixed culture with M. flocculare as demonstrated by PCR. By use of a M. flocculare antiserum it was possible to eliminate M. flocculare from M. hyopneumoniae mixed cultures. This study is the first report on isolation of porcine mycoplasmas at Gran Canaria (Spain).
    Original languageEnglish
    JournalVeterinary Research Communications
    Volume29
    Issue number6
    Pages (from-to)453-462
    ISSN0165-7380
    DOIs
    Publication statusPublished - 2005

    Keywords

    • diagnostic
    • porcine enzootic pneumonia
    • isolation
    • mycoplasma
    • PCR

    Cite this

    Assuncao, P., De la Fe, C., Kokotovic, B., Gonzalez, O., & Poveda, J. B. (2005). The occurrence of mycoplasmas in the lungs of swine in Gran Canaria (Spain). Veterinary Research Communications, 29(6), 453-462. https://doi.org/10.1007/s11259-005-1866-3