The need for transparency and good practices in the qPCR literature.

Stephen A Bustin; Vladimir Benes; Jeremy Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory Shipley; Carl T. Wittwer; Peter Schjerling; Philip J. Day; Mónica Abreu; Begoña Aguado; Jean-François Beaulieu; Anneleen Beckers; Sara Bogaert; John A. Browne; Fernando Carrasco-Ramiro; Liesbeth Ceelen; Kate Ciborowski; Pieter Cornillie; Stephanie Coulon; Ann Cuypers; Sara De Brouwer; Leentje De Ceuninck; Jurgen De Craene; Hélène De Naeyer; Ward De Spiegelaere; Kato Deckers; Annelies Dheedene; Kaat Durinck; Margarida Ferreira-Teixeira; Annelies Fieuw; Jack M Gallup; Sandra Gonzalo-Flores; Karen Goossens; Femke Heindryckx; Elizabeth Herring; Hans Hoenicka; Laura Icardi; Rolf Jaggi; Farzad Javad; Michael Karampelias; Frederick Kibenge; Molly Kibenge; Candy Kumps; Irina Lambertz; Tim Lammens; Amelia Markey; Peter Messiaen; Evelien Mets; Sofia Morais; Alberto Mudarra-Rubio; Justine Nakiwala; Hilde Nelis; Pal A Olsvik; Claudina Pérez-Novo; Michelle Plusquin; Tony Remans; Ali Rihani; Paulo Rodrigues-Santos; Pieter Rondou; Rebecca Sanders; Katharina Schmidt-Bleek; Kerstin Skovgaard; Karen Smeets; Laura Tabera; Stefan Toegel; Tim Van Acker; Wim Van den Broeck; Joni Van der Meulen; Mireille Van Gele; Gert Van Peer; Mario Van Poucke; Nadine Van Roy; Sarah Vergult; Joris Wauman; Marina Tshuikina-Wiklander; Erik Willems; Sara Zaccara; Fjoralba Zeka; Jo Vandesompele

doi:10.1038/nmeth.2697

The need for transparency and good practices in the qPCR literature.

Stephen A Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, Carl T. Wittwer, Peter Schjerling, Philip J. Day, Mónica Abreu, Begoña Aguado, Jean-François Beaulieu, Anneleen Beckers, Sara Bogaert, John A. Browne, Fernando Carrasco-RamiroLiesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Hélène De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michael Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A Olsvik, Claudina Pérez-Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, Jo Vandesompele

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Original language	English
Journal	Nature Methods
Volume	10
Issue number	11
Pages (from-to)	1063-1067
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.2697
Publication status	Published - 2013

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Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G., Wittwer, C. T., Schjerling, P., Day, P. J., Abreu, M., Aguado, B., Beaulieu, J-F., Beckers, A., Bogaert, S., Browne, J. A., ... Vandesompele, J. (2013). The need for transparency and good practices in the qPCR literature. Nature Methods, 10(11), 1063-1067. https://doi.org/10.1038/nmeth.2697

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abstract = "Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.",

author = "Bustin, {Stephen A} and Vladimir Benes and Jeremy Garson and Jan Hellemans and Jim Huggett and Mikael Kubista and Reinhold Mueller and Tania Nolan and Pfaffl, {Michael W} and Gregory Shipley and Wittwer, {Carl T.} and Peter Schjerling and Day, {Philip J.} and M{\'o}nica Abreu and Bego{\~n}a Aguado and Jean-Fran{\c c}ois Beaulieu and Anneleen Beckers and Sara Bogaert and Browne, {John A.} and Fernando Carrasco-Ramiro and Liesbeth Ceelen and Kate Ciborowski and Pieter Cornillie and Stephanie Coulon and Ann Cuypers and {De Brouwer}, Sara and {De Ceuninck}, Leentje and {De Craene}, Jurgen and {De Naeyer}, H{\'e}l{\`e}ne and {De Spiegelaere}, Ward and Kato Deckers and Annelies Dheedene and Kaat Durinck and Margarida Ferreira-Teixeira and Annelies Fieuw and Gallup, {Jack M} and Sandra Gonzalo-Flores and Karen Goossens and Femke Heindryckx and Elizabeth Herring and Hans Hoenicka and Laura Icardi and Rolf Jaggi and Farzad Javad and Michael Karampelias and Frederick Kibenge and Molly Kibenge and Candy Kumps and Irina Lambertz and Tim Lammens and Amelia Markey and Peter Messiaen and Evelien Mets and Sofia Morais and Alberto Mudarra-Rubio and Justine Nakiwala and Hilde Nelis and Olsvik, {Pal A} and Claudina P{\'e}rez-Novo and Michelle Plusquin and Tony Remans and Ali Rihani and Paulo Rodrigues-Santos and Pieter Rondou and Rebecca Sanders and Katharina Schmidt-Bleek and Kerstin Skovgaard and Karen Smeets and Laura Tabera and Stefan Toegel and {Van Acker}, Tim and {Van den Broeck}, Wim and {Van der Meulen}, Joni and {Van Gele}, Mireille and {Van Peer}, Gert and {Van Poucke}, Mario and {Van Roy}, Nadine and Sarah Vergult and Joris Wauman and Marina Tshuikina-Wiklander and Erik Willems and Sara Zaccara and Fjoralba Zeka and Jo Vandesompele",

year = "2013",

doi = "10.1038/nmeth.2697",

language = "English",

volume = "10",

pages = "1063--1067",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "11",

}

Bustin, SA, Benes, V, Garson, J, Hellemans, J, Huggett, J, Kubista, M, Mueller, R, Nolan, T, Pfaffl, MW, Shipley, G, Wittwer, CT, Schjerling, P, Day, PJ, Abreu, M, Aguado, B, Beaulieu, J-F, Beckers, A, Bogaert, S, Browne, JA, Carrasco-Ramiro, F, Ceelen, L, Ciborowski, K, Cornillie, P, Coulon, S, Cuypers, A, De Brouwer, S, De Ceuninck, L, De Craene, J, De Naeyer, H, De Spiegelaere, W, Deckers, K, Dheedene, A, Durinck, K, Ferreira-Teixeira, M, Fieuw, A, Gallup, JM, Gonzalo-Flores, S, Goossens, K, Heindryckx, F, Herring, E, Hoenicka, H, Icardi, L, Jaggi, R, Javad, F, Karampelias, M, Kibenge, F, Kibenge, M, Kumps, C, Lambertz, I, Lammens, T, Markey, A, Messiaen, P, Mets, E, Morais, S, Mudarra-Rubio, A, Nakiwala, J, Nelis, H, Olsvik, PA, Pérez-Novo, C, Plusquin, M, Remans, T, Rihani, A, Rodrigues-Santos, P, Rondou, P, Sanders, R, Schmidt-Bleek, K, Skovgaard, K, Smeets, K, Tabera, L, Toegel, S, Van Acker, T, Van den Broeck, W, Van der Meulen, J, Van Gele, M, Van Peer, G, Van Poucke, M, Van Roy, N, Vergult, S, Wauman, J, Tshuikina-Wiklander, M, Willems, E, Zaccara, S, Zeka, F & Vandesompele, J 2013, 'The need for transparency and good practices in the qPCR literature.', Nature Methods, vol. 10, no. 11, pp. 1063-1067. https://doi.org/10.1038/nmeth.2697

TY - JOUR

T1 - The need for transparency and good practices in the qPCR literature.

AU - Bustin, Stephen A

AU - Benes, Vladimir

AU - Garson, Jeremy

AU - Hellemans, Jan

AU - Huggett, Jim

AU - Kubista, Mikael

AU - Mueller, Reinhold

AU - Nolan, Tania

AU - Pfaffl, Michael W

AU - Shipley, Gregory

AU - Wittwer, Carl T.

AU - Schjerling, Peter

AU - Day, Philip J.

AU - Abreu, Mónica

AU - Aguado, Begoña

AU - Beaulieu, Jean-François

AU - Beckers, Anneleen

AU - Bogaert, Sara

AU - Browne, John A.

AU - Carrasco-Ramiro, Fernando

AU - Ceelen, Liesbeth

AU - Ciborowski, Kate

AU - Cornillie, Pieter

AU - Coulon, Stephanie

AU - Cuypers, Ann

AU - De Brouwer, Sara

AU - De Ceuninck, Leentje

AU - De Craene, Jurgen

AU - De Naeyer, Hélène

AU - De Spiegelaere, Ward

AU - Deckers, Kato

AU - Dheedene, Annelies

AU - Durinck, Kaat

AU - Ferreira-Teixeira, Margarida

AU - Fieuw, Annelies

AU - Gallup, Jack M

AU - Gonzalo-Flores, Sandra

AU - Goossens, Karen

AU - Heindryckx, Femke

AU - Herring, Elizabeth

AU - Hoenicka, Hans

AU - Icardi, Laura

AU - Jaggi, Rolf

AU - Javad, Farzad

AU - Karampelias, Michael

AU - Kibenge, Frederick

AU - Kibenge, Molly

AU - Kumps, Candy

AU - Lambertz, Irina

AU - Lammens, Tim

AU - Markey, Amelia

AU - Messiaen, Peter

AU - Mets, Evelien

AU - Morais, Sofia

AU - Mudarra-Rubio, Alberto

AU - Nakiwala, Justine

AU - Nelis, Hilde

AU - Olsvik, Pal A

AU - Pérez-Novo, Claudina

AU - Plusquin, Michelle

AU - Remans, Tony

AU - Rihani, Ali

AU - Rodrigues-Santos, Paulo

AU - Rondou, Pieter

AU - Sanders, Rebecca

AU - Schmidt-Bleek, Katharina

AU - Skovgaard, Kerstin

AU - Smeets, Karen

AU - Tabera, Laura

AU - Toegel, Stefan

AU - Van Acker, Tim

AU - Van den Broeck, Wim

AU - Van der Meulen, Joni

AU - Van Gele, Mireille

AU - Van Peer, Gert

AU - Van Poucke, Mario

AU - Van Roy, Nadine

AU - Vergult, Sarah

AU - Wauman, Joris

AU - Tshuikina-Wiklander, Marina

AU - Willems, Erik

AU - Zaccara, Sara

AU - Zeka, Fjoralba

AU - Vandesompele, Jo

PY - 2013

Y1 - 2013

N2 - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

AB - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

U2 - 10.1038/nmeth.2697

DO - 10.1038/nmeth.2697

M3 - Journal article

SN - 1548-7091

VL - 10

SP - 1063

EP - 1067

JO - Nature Methods

JF - Nature Methods

IS - 11

ER -

The need for transparency and good practices in the qPCR literature.

Abstract

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