TY - CHAP
T1 - The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
AU - Browne, Patrick Denis
AU - Kot, Witold
AU - Jørgensen, Tue Sparholt
AU - Hansen, Lars Hestbjerg
PY - 2020
Y1 - 2020
N2 - Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
AB - Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
U2 - 10.1007/978-1-4939-9877-7_18
DO - 10.1007/978-1-4939-9877-7_18
M3 - Book chapter
C2 - 31584168
VL - 2075
T3 - Methods in Molecular Biology
SP - 253
EP - 264
BT - Horizontal Gene Transfer
ER -