The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements

Patrick Denis Browne, Witold Kot, Tue Sparholt Jørgensen, Lars Hestbjerg Hansen

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Abstract

Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
Original languageEnglish
Title of host publicationHorizontal Gene Transfer
Volume2075
Publication date2020
Pages253-264
DOIs
Publication statusPublished - 2020
SeriesMethods in Molecular Biology
ISSN1064-3745

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