The mechanism of translation of cowpea mosaic-virus middle component RNA - no evidence for internal initiation from experiments in an animal-cell transient expression system

Graham Belsham, G. P. Lomonossoff

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The possibility that internal initiation of translation is responsible for the synthesis of the middle component (M) RNA-encoded 95K protein of cowpea mosaic virus (CPMV) has been investigated by constructing plasmids in which the entire sequence of CPMV M RNA was cloned downstream of a chloramphenicol acetyltransferase gene. Expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream CPMV open reading frame takes place from RNA derived from these constructs under conditions where the internal ribosome entry site of foot-and-mouth disease virus is functional. The results indicate that internal initiation is not responsible for the synthesis of the 95K protein in this system.
Original languageEnglish
JournalJournal of General Virology
Volume72
Issue numberPart 12
Pages (from-to)3109-3113
ISSN0022-1317
DOIs
Publication statusPublished - 1991
Externally publishedYes

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