The Kinetics, Thermodynamics and Mechanisms of Short Aromatic Peptide Self-Assembly

Thomas O. Mason, Alexander K. Buell*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The self-assembly of short aromatic peptides and peptide derivatives into a variety of different nano- and microstructures (fibrillar gels, crystals, spheres, plates) is a promising route toward the creation of bio-compatible materials with often unexpected and useful properties. Furthermore, such simple self-assembling systems have been proposed as model systems for the self-assembly of longer peptides, a process that can be linked to biological function and malfunction. Much effort has been made in the last 15 years to explore the space of peptide sequences, chemical modifications and solvent conditions in order to maximise the diversity of assembly morphologies and properties. However, quantitative studies of the corresponding mechanisms of, and driving forces for, peptide self-assembly have remained relatively scarce until recently. In this chapter we review the current state of understanding of the thermodynamic driving forces and self-assembly mechanisms of short aromatic peptides into supramolecular structures. We will focus on experimental studies of the assembly process and our perspective will be centered around diphenylalanine (FF), a key motif of the amyloid β sequence and a paradigmatic self-assembly building block. Our main focus is the basic physical chemistry and key structural aspects of such systems, and we will also compare the mechanism of dipeptide aggregation with that of longer peptide sequences into amyloid fibrils, with discussion on how these mechanisms may be revealed through detailed analysis of growth kinetics, thermodynamics and other fundamental properties of the aggregation process.
Original languageEnglish
Title of host publicationBiological and Bio-inspired Nanomaterials
Number of pages52
PublisherSpringer
Publication date2019
Pages61-112
Chapter3
ISBN (Print)978-981-13-9790-5
ISBN (Electronic)978-981-13-9791-2
DOIs
Publication statusPublished - 2019
SeriesAdvances in Experimental medicine and Biology
Volume1174
ISSN0065-2598

Keywords

  • FF
  • Aromaticity
  • Amyloid
  • Self-assembly
  • Crystal
  • Fibril
  • Gel
  • Nucleation
  • Microfluidics
  • Biomaterials

Cite this

Mason, T. O., & Buell, A. K. (2019). The Kinetics, Thermodynamics and Mechanisms of Short Aromatic Peptide Self-Assembly. In Biological and Bio-inspired Nanomaterials (pp. 61-112). Springer. Advances in Experimental medicine and Biology, Vol.. 1174 https://doi.org/10.1007/978-981-13-9791-2_3