The interplay of sequence conservation and T cell immune recognition

Anne Gøther Bresciani, Alessandro Sette, Jason Greenbaum, Morten Nielsen, Cecilia S Lindestam Arlehamn, Bjoern Peters

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    223 Downloads (Pure)

    Abstract

    Predicting which peptides can elicit a T cell response (i.e. are immunogenic) is of great importance for many immunological studies. While it is clear that MHC binding is a necessary requirement for peptide immunogenicity, other variables exist that are incompletely understood. In this study we examined the hypothesis that conservation of a peptide in bacteria that are part of the healthy human microbiome leads to a reduced level of immunogenicity due to tolerization of T cells to the commensal bacteria. This was done by comparing experimentally characterized T cell epitope recognition data from the Immune Epitope Database with their conservation in the human microbiome. Indeed, we did see a lower immunogenicity for conserved peptides conserved. While many aspects how this conservation comparison is done require further optimization, this is a first step towards a better understanding T cell recognition of peptides in bacterial pathogens is influenced by their conservation in commensal bacteria. If the further work proves that this approach is successful, the degree of overlap of a peptide with the human proteome or microbiome could be added to the arsenal of tools available to assess peptide immunogenicity.
    Original languageEnglish
    Title of host publicationProceedings of the 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics
    Number of pages5
    Place of PublicationNew York
    PublisherAssociation for Computing Machinery
    Publication date2014
    Pages739-743
    ISBN (Print)978-1-4503-2894-4
    DOIs
    Publication statusPublished - 2014
    EventThe 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics: BCB '14 - Newport Beach, CA, United States
    Duration: 20 Sep 201423 Sep 2014
    Conference number: 5

    Conference

    ConferenceThe 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics
    Number5
    Country/TerritoryUnited States
    CityNewport Beach, CA
    Period20/09/201423/09/2014

    Keywords

    • Immune epitope database
    • Human microbiome
    • Epitopes
    • Sequence conservation
    • Immunogenicity

    Fingerprint

    Dive into the research topics of 'The interplay of sequence conservation and T cell immune recognition'. Together they form a unique fingerprint.

    Cite this