TY - JOUR
T1 - The influence of salt type on the retention of bovine serum albumin in ion-exchange chromatography
AU - Al-Jibbouri, Sattar
PY - 2007
Y1 - 2007
N2 - In this paper, an analysis of the influence of the salt types, NaCl, NaCH"3COO, Na"2SO"4 and Na"3C"6H"5O"7, on the isocratic retention behaviour of bovine serum albumin (BSA) on two anion-exchangers media (Source 30Q and TSK Gel Super Q 5 PW) has been presented. The retention data demonstrated that the mechanism of protein retention in ion-exchange chromatography (IEC) involves interactions between the protein solute, the mobile phase constituents and the stationary phase. The effect of protein activity coefficient in the mobile phase on the protein retention volumes is verified.
AB - In this paper, an analysis of the influence of the salt types, NaCl, NaCH"3COO, Na"2SO"4 and Na"3C"6H"5O"7, on the isocratic retention behaviour of bovine serum albumin (BSA) on two anion-exchangers media (Source 30Q and TSK Gel Super Q 5 PW) has been presented. The retention data demonstrated that the mechanism of protein retention in ion-exchange chromatography (IEC) involves interactions between the protein solute, the mobile phase constituents and the stationary phase. The effect of protein activity coefficient in the mobile phase on the protein retention volumes is verified.
U2 - 10.1016/j.chroma.2006.10.079
DO - 10.1016/j.chroma.2006.10.079
M3 - Journal article
C2 - 17109867
SN - 0021-9673
VL - 1139
SP - 57
EP - 62
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -