The Incidence of Necrotizing Enterocolitis Is Increased Following Probiotic Administration to Preterm Pigs

Malene Skovsted Cilieborg, Thomas Thymann, Richard Siggers, Mette Boye, Stine B. Bering, Bent B. Jensen, Per T. Sangild

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Preterm birth and necrotizing enterocolitis (NEC) is associated with inappropriate gut colonization and immunity, which may be improved by probiotic bacteria. Using a preterm pig model of NEC, we investigated the effects of probiotics on intestinal structure, function, microbiology, and immunology in the immediate postnatal period. Just after birth, caesarean-delivered preterm pigs were inoculated with Lactobacillus paracasei, Bifidobacteria animalis, and Streptococcus thermophilus (total 2.4 x 1010/d) either as live (ProLive, n = 14) or gamma-irradiated dead bacteria (ProDead, n = 12) and compared with controls (n = 14). All pigs received parenteral nutrition for 2 d followed by enteral formula feeding until tissue collection on d 5. Compared with control pigs, intestinal weight was lower and NEC incidence was higher in both groups given probiotics (64–67 vs. 14%; P
    Original languageEnglish
    JournalJournal of Nutrition
    Volume141
    Issue number2
    Pages (from-to)223-230
    ISSN0022-3166
    DOIs
    Publication statusPublished - 2011

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