The impact of screening-test negative samples not enumerated by MPN

Luis Gustavo Corbellini, Ana Sofia Ribeiro Duarte, Leonardo de Knegt, Luis Eduardo da Silva, Marisa Cardoso, Maarten Nauta

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In microbiological surveys, false negative results in detection tests precluding the enumeration by MPN may occur. The objective of this study was to illustrate the impact of screening test failure on the probability distribution of Salmonella concentrations in pork using a Bayesian method. A total of 276 swab samples in four slaughter steps (69 samples in each slaughter step: after dehairing, after singeing, after evisceration, and before chilling) were screened for Salmonella and enumerated by the MPN method. Salmonella contamination data were fitted to a lognormal distribution by using a Bayesian model that uses the number of positive tubes at each dilution in an MPN analysis to estimate the parameters of the concentration distribution. With Salmonella paired data, three data sets were used for each slaughter step: one that includes the positives in the screening test only, a second one that includes false negative results from the screening, and a third that considers the entire data set. The relative sensitivity of the screening test was also calculated assuming as gold standard samples with confirmed Salmonella. Salmonella was confirmed by a reference laboratory in 29 samples either by screening or MPN method. The relative sensitivity of the screening test was 69% (CI 95%: 52%–85%). The data set that included enumerations from screen-negative samples (false negative results) tended to have higher View the MathML sourceμ^ and smaller View the MathML sourceσ^ in comparison with the data set that discards false negative results, suggesting that the lack of sensitivity of the screening test affects the distribution that describes the contamination across the population. Numerous surveys on fitting distribution methods of microbial censored data have been published and discuss source of bias due to fitting method. Results of this survey contribute with that discussion by illustrating another possible source of bias due to failure of the screening methods preceding the MPN.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume205
Pages (from-to)1-6
ISSN0168-1605
DOIs
Publication statusPublished - 2015

Keywords

  • Concentration distribution
  • Bayesian
  • Food safety
  • Salmonella
  • Pork
  • Quantitative microbiological risk assessment

Cite this

Corbellini, Luis Gustavo ; Ribeiro Duarte, Ana Sofia ; de Knegt, Leonardo ; Silva, Luis Eduardo da ; Cardoso, Marisa ; Nauta, Maarten. / The impact of screening-test negative samples not enumerated by MPN. In: International Journal of Food Microbiology. 2015 ; Vol. 205. pp. 1-6.
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title = "The impact of screening-test negative samples not enumerated by MPN",
abstract = "In microbiological surveys, false negative results in detection tests precluding the enumeration by MPN may occur. The objective of this study was to illustrate the impact of screening test failure on the probability distribution of Salmonella concentrations in pork using a Bayesian method. A total of 276 swab samples in four slaughter steps (69 samples in each slaughter step: after dehairing, after singeing, after evisceration, and before chilling) were screened for Salmonella and enumerated by the MPN method. Salmonella contamination data were fitted to a lognormal distribution by using a Bayesian model that uses the number of positive tubes at each dilution in an MPN analysis to estimate the parameters of the concentration distribution. With Salmonella paired data, three data sets were used for each slaughter step: one that includes the positives in the screening test only, a second one that includes false negative results from the screening, and a third that considers the entire data set. The relative sensitivity of the screening test was also calculated assuming as gold standard samples with confirmed Salmonella. Salmonella was confirmed by a reference laboratory in 29 samples either by screening or MPN method. The relative sensitivity of the screening test was 69{\%} (CI 95{\%}: 52{\%}–85{\%}). The data set that included enumerations from screen-negative samples (false negative results) tended to have higher View the MathML sourceμ^ and smaller View the MathML sourceσ^ in comparison with the data set that discards false negative results, suggesting that the lack of sensitivity of the screening test affects the distribution that describes the contamination across the population. Numerous surveys on fitting distribution methods of microbial censored data have been published and discuss source of bias due to fitting method. Results of this survey contribute with that discussion by illustrating another possible source of bias due to failure of the screening methods preceding the MPN.",
keywords = "Concentration distribution, Bayesian, Food safety, Salmonella, Pork, Quantitative microbiological risk assessment",
author = "Corbellini, {Luis Gustavo} and {Ribeiro Duarte}, {Ana Sofia} and {de Knegt}, Leonardo and Silva, {Luis Eduardo da} and Marisa Cardoso and Maarten Nauta",
year = "2015",
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language = "English",
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The impact of screening-test negative samples not enumerated by MPN. / Corbellini, Luis Gustavo; Ribeiro Duarte, Ana Sofia; de Knegt, Leonardo; Silva, Luis Eduardo da; Cardoso, Marisa; Nauta, Maarten.

In: International Journal of Food Microbiology, Vol. 205, 2015, p. 1-6.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The impact of screening-test negative samples not enumerated by MPN

AU - Corbellini, Luis Gustavo

AU - Ribeiro Duarte, Ana Sofia

AU - de Knegt, Leonardo

AU - Silva, Luis Eduardo da

AU - Cardoso, Marisa

AU - Nauta, Maarten

PY - 2015

Y1 - 2015

N2 - In microbiological surveys, false negative results in detection tests precluding the enumeration by MPN may occur. The objective of this study was to illustrate the impact of screening test failure on the probability distribution of Salmonella concentrations in pork using a Bayesian method. A total of 276 swab samples in four slaughter steps (69 samples in each slaughter step: after dehairing, after singeing, after evisceration, and before chilling) were screened for Salmonella and enumerated by the MPN method. Salmonella contamination data were fitted to a lognormal distribution by using a Bayesian model that uses the number of positive tubes at each dilution in an MPN analysis to estimate the parameters of the concentration distribution. With Salmonella paired data, three data sets were used for each slaughter step: one that includes the positives in the screening test only, a second one that includes false negative results from the screening, and a third that considers the entire data set. The relative sensitivity of the screening test was also calculated assuming as gold standard samples with confirmed Salmonella. Salmonella was confirmed by a reference laboratory in 29 samples either by screening or MPN method. The relative sensitivity of the screening test was 69% (CI 95%: 52%–85%). The data set that included enumerations from screen-negative samples (false negative results) tended to have higher View the MathML sourceμ^ and smaller View the MathML sourceσ^ in comparison with the data set that discards false negative results, suggesting that the lack of sensitivity of the screening test affects the distribution that describes the contamination across the population. Numerous surveys on fitting distribution methods of microbial censored data have been published and discuss source of bias due to fitting method. Results of this survey contribute with that discussion by illustrating another possible source of bias due to failure of the screening methods preceding the MPN.

AB - In microbiological surveys, false negative results in detection tests precluding the enumeration by MPN may occur. The objective of this study was to illustrate the impact of screening test failure on the probability distribution of Salmonella concentrations in pork using a Bayesian method. A total of 276 swab samples in four slaughter steps (69 samples in each slaughter step: after dehairing, after singeing, after evisceration, and before chilling) were screened for Salmonella and enumerated by the MPN method. Salmonella contamination data were fitted to a lognormal distribution by using a Bayesian model that uses the number of positive tubes at each dilution in an MPN analysis to estimate the parameters of the concentration distribution. With Salmonella paired data, three data sets were used for each slaughter step: one that includes the positives in the screening test only, a second one that includes false negative results from the screening, and a third that considers the entire data set. The relative sensitivity of the screening test was also calculated assuming as gold standard samples with confirmed Salmonella. Salmonella was confirmed by a reference laboratory in 29 samples either by screening or MPN method. The relative sensitivity of the screening test was 69% (CI 95%: 52%–85%). The data set that included enumerations from screen-negative samples (false negative results) tended to have higher View the MathML sourceμ^ and smaller View the MathML sourceσ^ in comparison with the data set that discards false negative results, suggesting that the lack of sensitivity of the screening test affects the distribution that describes the contamination across the population. Numerous surveys on fitting distribution methods of microbial censored data have been published and discuss source of bias due to fitting method. Results of this survey contribute with that discussion by illustrating another possible source of bias due to failure of the screening methods preceding the MPN.

KW - Concentration distribution

KW - Bayesian

KW - Food safety

KW - Salmonella

KW - Pork

KW - Quantitative microbiological risk assessment

U2 - 10.1016/j.ijfoodmicro.2015.03.023

DO - 10.1016/j.ijfoodmicro.2015.03.023

M3 - Journal article

C2 - 25866905

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EP - 6

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

ER -