The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae

José Luis Martinez Ruiz, Eugenio Meza, Dina Petranovic, Jens Nielsen

Research output: Contribution to journalJournal articleResearchpeer-review

355 Downloads (Pure)

Abstract

Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purify a secreted product. However, recombinant production at high rates represents a significant metabolic burden for the yeast cells, which results in oxidative stress and ultimately affects the protein production capacity. Here we describe a method to reduce the overall oxidative stress by overexpressing the endogenous HAP1 gene in a S. cerevisiae strain overproducing recombinant α-amylase. We demonstrate how Hap1p can activate a set of oxidative stress response genes and meanwhile contribute to increase the metabolic rate of the yeast strains, therefore mitigating the negative effect of the ROS accumulation associated to protein folding and hence increasing the production capacity during batch fermentations.
Original languageEnglish
JournalMetabolic Engineering Communications
Volume3
Pages (from-to)205-210
Number of pages6
ISSN2214-0301
DOIs
Publication statusPublished - 2016

Bibliographical note

Under a Creative Commons license

Keywords

  • Amylase
  • Hap1
  • Oxidative stress response
  • Protein production
  • Yeast

Fingerprint Dive into the research topics of 'The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this