TY - JOUR
T1 - The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till
AU - Bælum, Jacob
AU - Scheutz, Charlotte
AU - Chambon, Julie Claire Claudia
AU - Jensen, Christine Mosegaard
AU - Brochmann, Rikke P.
AU - Dennis, Philip
AU - Laier, Troels
AU - Broholm, Mette Martina
AU - Bjerg, Poul Løgstrup
AU - Binning, Philip John
AU - Jacobsen, Carsten
PY - 2014
Y1 - 2014
N2 - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination. © 2013 Elsevier Ltd. All rights reserved.
AB - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination. © 2013 Elsevier Ltd. All rights reserved.
KW - Chlorine compounds
KW - Ethylene
KW - Genes
KW - Models
KW - Polymerase chain reaction
KW - Dechlorination
U2 - 10.1016/j.envpol.2013.11.013
DO - 10.1016/j.envpol.2013.11.013
M3 - Journal article
C2 - 24374375
SN - 0269-7491
VL - 186
SP - 149
EP - 157
JO - Environmental Pollution
JF - Environmental Pollution
ER -