The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

Jacob Bælum, Charlotte Scheutz, Julie Claire Claudia Chambon, Christine Mosegaard Jensen, Rikke P. Brochmann, Philip Dennis, Troels Laier, Mette Martina Broholm, Poul Løgstrup Bjerg, Philip John Binning, Carsten Jacobsen

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Abstract

A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination. © 2013 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalEnvironmental Pollution
Volume186
Pages (from-to)149-157
Number of pages9
ISSN0269-7491
DOIs
Publication statusPublished - 2014

Keywords

  • Chlorine compounds
  • Ethylene
  • Genes
  • Models
  • Polymerase chain reaction
  • Dechlorination

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