The immune response of rainbow trout to Flavobacterium psychrophilum following immersion-challenge model with and without hydrogen peroxide pre-treatment

Maya Maria Mihályi Henriksen, Lone Madsen, P. W. Kania, K. Buchmann, Inger Dalsgaard

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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    Abstract

    The bacterial fish pathogen Flavobacterium psychrophilum is a major cause of mortality in farmed rainbow trout (Oncorhynchus mykiss) and other salmonid fish. The disease following infection is called bacterial coldwater disease (BCWD) or rainbow trout fry syndrome (RTFS). To our knowledge, no commercial vaccine is currently available and the disease is treated with antibiotics. Injection-based challenges with F. psychrophilum are standardized but the route of infection does not reflect a natural situation. Therefore, we established an immersion-based model investigating if hydrogen peroxide (H2O2) pre-treatment could elevate infection and mortality.

    The model consisted of four groups: 1) Un-exposed control, 2) H2O2 exposure, 3) F. psychrophilum immersion and 4) H2O2 + F. psychrophilum. Pre-treatment with H2O2 increased mortality two-fold if fish also were exposed to F. psychrophilum after pretreatment. Tissue samples were taken from the involved groups 4 h, 48 h, 125 h and 192 h post-exposure and investigated for regulation of immune genes. Following genes were examined in the head kidney and gills by qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-6, IL-1β, IL-17, SAA and FoxP3.

    A pro-inflammatory response was indicated, but only a weak indication of an adaptive response was recorded (most evident in the F. psychrophilum group). Further, pre-treatment with H2O2 affected the correlation gene expression and pathogen load in several cases. Morphological changes in the
    gill tissue were evaluated using hematoxylin and eosin stained tissue sections. Exposure to both H2O2 and F. psychrophilum intensified tissue damage and postponed healing. The results indicate that F. psychrophilum may have an immunosuppressive action and that environmental stress may be one of several factors playing a role in RTFS outbreaks.
    Original languageEnglish
    Title of host publicationDAFINET Workshop and PhD Course : Book of Abstracts
    Number of pages1
    Publication date2013
    Publication statusPublished - 2013
    EventDAFINET Workshop : Fish Immunology: From Egg to Adult Fish - Frederiksberg, Denmark
    Duration: 12 Nov 201314 Nov 2013
    http://www.dafinet.dk

    Conference

    ConferenceDAFINET Workshop : Fish Immunology: From Egg to Adult Fish
    CountryDenmark
    CityFrederiksberg
    Period12/11/201314/11/2013
    Internet address

    Cite this

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    title = "The immune response of rainbow trout to Flavobacterium psychrophilum following immersion-challenge model with and without hydrogen peroxide pre-treatment",
    abstract = "The bacterial fish pathogen Flavobacterium psychrophilum is a major cause of mortality in farmed rainbow trout (Oncorhynchus mykiss) and other salmonid fish. The disease following infection is called bacterial coldwater disease (BCWD) or rainbow trout fry syndrome (RTFS). To our knowledge, no commercial vaccine is currently available and the disease is treated with antibiotics. Injection-based challenges with F. psychrophilum are standardized but the route of infection does not reflect a natural situation. Therefore, we established an immersion-based model investigating if hydrogen peroxide (H2O2) pre-treatment could elevate infection and mortality.The model consisted of four groups: 1) Un-exposed control, 2) H2O2 exposure, 3) F. psychrophilum immersion and 4) H2O2 + F. psychrophilum. Pre-treatment with H2O2 increased mortality two-fold if fish also were exposed to F. psychrophilum after pretreatment. Tissue samples were taken from the involved groups 4 h, 48 h, 125 h and 192 h post-exposure and investigated for regulation of immune genes. Following genes were examined in the head kidney and gills by qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-6, IL-1β, IL-17, SAA and FoxP3. A pro-inflammatory response was indicated, but only a weak indication of an adaptive response was recorded (most evident in the F. psychrophilum group). Further, pre-treatment with H2O2 affected the correlation gene expression and pathogen load in several cases. Morphological changes in thegill tissue were evaluated using hematoxylin and eosin stained tissue sections. Exposure to both H2O2 and F. psychrophilum intensified tissue damage and postponed healing. The results indicate that F. psychrophilum may have an immunosuppressive action and that environmental stress may be one of several factors playing a role in RTFS outbreaks.",
    author = "Henriksen, {Maya Maria Mih{\'a}lyi} and Lone Madsen and Kania, {P. W.} and K. Buchmann and Inger Dalsgaard",
    year = "2013",
    language = "English",
    booktitle = "DAFINET Workshop and PhD Course",

    }

    Henriksen, MMM, Madsen, L, Kania, PW, Buchmann, K & Dalsgaard, I 2013, The immune response of rainbow trout to Flavobacterium psychrophilum following immersion-challenge model with and without hydrogen peroxide pre-treatment. in DAFINET Workshop and PhD Course: Book of Abstracts. DAFINET Workshop : Fish Immunology: From Egg to Adult Fish, Frederiksberg, Denmark, 12/11/2013.

    The immune response of rainbow trout to Flavobacterium psychrophilum following immersion-challenge model with and without hydrogen peroxide pre-treatment. / Henriksen, Maya Maria Mihályi; Madsen, Lone; Kania, P. W.; Buchmann, K.; Dalsgaard, Inger.

    DAFINET Workshop and PhD Course: Book of Abstracts. 2013.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    TY - ABST

    T1 - The immune response of rainbow trout to Flavobacterium psychrophilum following immersion-challenge model with and without hydrogen peroxide pre-treatment

    AU - Henriksen, Maya Maria Mihályi

    AU - Madsen, Lone

    AU - Kania, P. W.

    AU - Buchmann, K.

    AU - Dalsgaard, Inger

    PY - 2013

    Y1 - 2013

    N2 - The bacterial fish pathogen Flavobacterium psychrophilum is a major cause of mortality in farmed rainbow trout (Oncorhynchus mykiss) and other salmonid fish. The disease following infection is called bacterial coldwater disease (BCWD) or rainbow trout fry syndrome (RTFS). To our knowledge, no commercial vaccine is currently available and the disease is treated with antibiotics. Injection-based challenges with F. psychrophilum are standardized but the route of infection does not reflect a natural situation. Therefore, we established an immersion-based model investigating if hydrogen peroxide (H2O2) pre-treatment could elevate infection and mortality.The model consisted of four groups: 1) Un-exposed control, 2) H2O2 exposure, 3) F. psychrophilum immersion and 4) H2O2 + F. psychrophilum. Pre-treatment with H2O2 increased mortality two-fold if fish also were exposed to F. psychrophilum after pretreatment. Tissue samples were taken from the involved groups 4 h, 48 h, 125 h and 192 h post-exposure and investigated for regulation of immune genes. Following genes were examined in the head kidney and gills by qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-6, IL-1β, IL-17, SAA and FoxP3. A pro-inflammatory response was indicated, but only a weak indication of an adaptive response was recorded (most evident in the F. psychrophilum group). Further, pre-treatment with H2O2 affected the correlation gene expression and pathogen load in several cases. Morphological changes in thegill tissue were evaluated using hematoxylin and eosin stained tissue sections. Exposure to both H2O2 and F. psychrophilum intensified tissue damage and postponed healing. The results indicate that F. psychrophilum may have an immunosuppressive action and that environmental stress may be one of several factors playing a role in RTFS outbreaks.

    AB - The bacterial fish pathogen Flavobacterium psychrophilum is a major cause of mortality in farmed rainbow trout (Oncorhynchus mykiss) and other salmonid fish. The disease following infection is called bacterial coldwater disease (BCWD) or rainbow trout fry syndrome (RTFS). To our knowledge, no commercial vaccine is currently available and the disease is treated with antibiotics. Injection-based challenges with F. psychrophilum are standardized but the route of infection does not reflect a natural situation. Therefore, we established an immersion-based model investigating if hydrogen peroxide (H2O2) pre-treatment could elevate infection and mortality.The model consisted of four groups: 1) Un-exposed control, 2) H2O2 exposure, 3) F. psychrophilum immersion and 4) H2O2 + F. psychrophilum. Pre-treatment with H2O2 increased mortality two-fold if fish also were exposed to F. psychrophilum after pretreatment. Tissue samples were taken from the involved groups 4 h, 48 h, 125 h and 192 h post-exposure and investigated for regulation of immune genes. Following genes were examined in the head kidney and gills by qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-6, IL-1β, IL-17, SAA and FoxP3. A pro-inflammatory response was indicated, but only a weak indication of an adaptive response was recorded (most evident in the F. psychrophilum group). Further, pre-treatment with H2O2 affected the correlation gene expression and pathogen load in several cases. Morphological changes in thegill tissue were evaluated using hematoxylin and eosin stained tissue sections. Exposure to both H2O2 and F. psychrophilum intensified tissue damage and postponed healing. The results indicate that F. psychrophilum may have an immunosuppressive action and that environmental stress may be one of several factors playing a role in RTFS outbreaks.

    M3 - Conference abstract in proceedings

    BT - DAFINET Workshop and PhD Course

    ER -