The identification of a tetracycline resistance gene tet(M), on a Tn916-like transposon, in the Bacillus cereus group

Yvonne Agersø, Lars Bogø Jensen, Michael Christian Givskov, M.C. Roberts

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In order to investigate whether resistance genes present in bacteria in manure could transfer to indigenous soil bacteria, resistant isolates belonging to the Bacillus cereus group (Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis) were isolated from farm soil (72 isolates) and manure (12 isolates) samples. These isolates were screened for tetracycline resistance genes (tet(K), tet(L), tet(M), tet(O), tet(S) and tet(T)). Of 88 isolates examined, three (3.4%) isolates carried both tet(M) and tet(L) genes, while four (4.5%) isolates carried the tet(L) gene. Eighty-one (92.1%) isolates did not contain any of the tested genes. All tet(M) positive isolates carried transposon Tn916 and could transfer this mobile DNA element to other Gram-positive bacteria.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume214
Issue number2
Pages (from-to)251-256
ISSN0378-1097
DOIs
Publication statusPublished - 2002

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