The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling

Cecilia Lindskog, Jerker Linne, Linn Fagerberg, Bjorn M. Hallstrom, Carl Johan Sundberg, Malene Lindholm, Mikael Huss, Caroline Kampf, Howard Choi, David A. Liem, Peipei Ping, Leif Väremo, Adil Mardinoglu, Jens Nielsen, Erik Larsson, Fredrik Ponten, Mathias Uhlén

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Background: To understand cardiac and skeletal muscle function, it is important to define and explore their molecular constituents and also to identify similarities and differences in the gene expression in these two different striated muscle tissues. Here, we have investigated the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs using a global transcriptomics analysis complemented with antibody-based profiling to localize the corresponding proteins on a single cell level.Results: Our study identified a comprehensive list of genes expressed in cardiac and skeletal muscle. The genes with elevated expression were further stratified according to their global expression pattern across the human body as well as their precise localization in the muscle tissues. The functions of the proteins encoded by the elevated genes are well in line with the physiological functions of cardiac and skeletal muscle, such as contraction, ion transport, regulation of membrane potential and actomyosin structure organization. A large fraction of the transcripts in both cardiac and skeletal muscle correspond to mitochondrial proteins involved in energy metabolism, which demonstrates the extreme specialization of these muscle tissues to provide energy for contraction.Conclusions: Our results provide a comprehensive list of genes and proteins elevated in striated muscles. A number of proteins not previously characterized in cardiac and skeletal muscle were identified and localized to specific cellular subcompartments. These proteins represent an interesting starting point for further functional analysis of their role in muscle biology and disease.
Original languageEnglish
Article number475
JournalBMC Genomics
Number of pages16
Publication statusPublished - 2015

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​4.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.


  • Transcriptome
  • Proteome
  • Cardiac and skeletal muscle

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