The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate

Steen Lyders Lerche Hansen, Jan Martinussen

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearch

    Abstract

    Nucleotide metabolism is important for all cells as supplier of building blocks for the synthesis of nucleic acids and coenzymes. Furthermore, they act as intracellular energy carriers and allosteric effectors in a large number of enzymatic reactions. Nucleotides can either be made de novo or from preformed metabolites present in the growth medium. In addition to the obvious phenotype of a mutant in the pyrimidine biosynthetic pathway - requirement for a pyrimidine precursor in the growth medium - pleiotrophic effects are often seen. The work presented here shows, that a mutation in one of the genes encoding enzymes in the distal part of the pyrimidine biosynthetic pathway of L. lactis MG1363, results in reduction of the growth rate if exogenous aspartate is supplied to the growth medium. This observation can be explained by an increased accumulation of a toxic intermediate, most likely carbamoyl aspartate, provoked by high concentrations of aspartate.
    Original languageEnglish
    Title of host publicationASM Conference on Streptococcal Genetics
    Place of PublicationWashington
    PublisherAmerican Society for Microbiology
    Publication date1998
    Pages68-68
    Publication statusPublished - 1998
    Event5th ASM Conference on Streptococcal Genetics - Vichy, France
    Duration: 26 Aug 199829 Aug 1998
    Conference number: 5

    Conference

    Conference5th ASM Conference on Streptococcal Genetics
    Number5
    CountryFrance
    CityVichy
    Period26/08/199829/08/1998

    Cite this

    Hansen, S. L. L., & Martinussen, J. (1998). The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate. In ASM Conference on Streptococcal Genetics (pp. 68-68). Washington: American Society for Microbiology.
    Hansen, Steen Lyders Lerche ; Martinussen, Jan. / The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate. ASM Conference on Streptococcal Genetics. Washington : American Society for Microbiology, 1998. pp. 68-68
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    title = "The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate",
    abstract = "Nucleotide metabolism is important for all cells as supplier of building blocks for the synthesis of nucleic acids and coenzymes. Furthermore, they act as intracellular energy carriers and allosteric effectors in a large number of enzymatic reactions. Nucleotides can either be made de novo or from preformed metabolites present in the growth medium. In addition to the obvious phenotype of a mutant in the pyrimidine biosynthetic pathway - requirement for a pyrimidine precursor in the growth medium - pleiotrophic effects are often seen. The work presented here shows, that a mutation in one of the genes encoding enzymes in the distal part of the pyrimidine biosynthetic pathway of L. lactis MG1363, results in reduction of the growth rate if exogenous aspartate is supplied to the growth medium. This observation can be explained by an increased accumulation of a toxic intermediate, most likely carbamoyl aspartate, provoked by high concentrations of aspartate.",
    author = "Hansen, {Steen Lyders Lerche} and Jan Martinussen",
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    Hansen, SLL & Martinussen, J 1998, The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate. in ASM Conference on Streptococcal Genetics. American Society for Microbiology, Washington, pp. 68-68, 5th ASM Conference on Streptococcal Genetics, Vichy, France, 26/08/1998.

    The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate. / Hansen, Steen Lyders Lerche; Martinussen, Jan.

    ASM Conference on Streptococcal Genetics. Washington : American Society for Microbiology, 1998. p. 68-68.

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearch

    TY - GEN

    T1 - The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate

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    PY - 1998

    Y1 - 1998

    N2 - Nucleotide metabolism is important for all cells as supplier of building blocks for the synthesis of nucleic acids and coenzymes. Furthermore, they act as intracellular energy carriers and allosteric effectors in a large number of enzymatic reactions. Nucleotides can either be made de novo or from preformed metabolites present in the growth medium. In addition to the obvious phenotype of a mutant in the pyrimidine biosynthetic pathway - requirement for a pyrimidine precursor in the growth medium - pleiotrophic effects are often seen. The work presented here shows, that a mutation in one of the genes encoding enzymes in the distal part of the pyrimidine biosynthetic pathway of L. lactis MG1363, results in reduction of the growth rate if exogenous aspartate is supplied to the growth medium. This observation can be explained by an increased accumulation of a toxic intermediate, most likely carbamoyl aspartate, provoked by high concentrations of aspartate.

    AB - Nucleotide metabolism is important for all cells as supplier of building blocks for the synthesis of nucleic acids and coenzymes. Furthermore, they act as intracellular energy carriers and allosteric effectors in a large number of enzymatic reactions. Nucleotides can either be made de novo or from preformed metabolites present in the growth medium. In addition to the obvious phenotype of a mutant in the pyrimidine biosynthetic pathway - requirement for a pyrimidine precursor in the growth medium - pleiotrophic effects are often seen. The work presented here shows, that a mutation in one of the genes encoding enzymes in the distal part of the pyrimidine biosynthetic pathway of L. lactis MG1363, results in reduction of the growth rate if exogenous aspartate is supplied to the growth medium. This observation can be explained by an increased accumulation of a toxic intermediate, most likely carbamoyl aspartate, provoked by high concentrations of aspartate.

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    SP - 68

    EP - 68

    BT - ASM Conference on Streptococcal Genetics

    PB - American Society for Microbiology

    CY - Washington

    ER -

    Hansen SLL, Martinussen J. The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate. In ASM Conference on Streptococcal Genetics. Washington: American Society for Microbiology. 1998. p. 68-68