The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

Xun Xu, Shengkai Pan, Xin Liu, Wenbin Chen, Min Xie, Wenliang Wang, Jun Wang, Harish Nagarajan, Nathan Lewis, Iman Famili, Bernhard O. Palsson, Zhiming Cai, Yaoting Gui, Stephanie Hammond, Kelvin H. Lee, Mikael Rørdam Andersen, Norma Neff, Benedetto Passarelli, Winston Koh, H. Christina FanJianbin Wang, Stephen R. Quake, Michael Betenbaugh, Bernhard Palsson, Jun Wang

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.
Original languageEnglish
JournalNature Biotechnology
Volume29
Issue number8
Pages (from-to)735-741
ISSN1087-0156
DOIs
Publication statusPublished - 2011

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