The gef gene from Escherichia coli is regulated at the level of translation

Lars K. Poulsen, Annette Refn, Søren Molin, Poul Andersson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We describe post-transcriptional regulation of the chromosomal gene, gef, from Escherichia coli. The gef gene is a member of a gene family consisting of the chromosomal gef and reIF genes from Escherichia coli and the hok, flmA, srnB, and pndA genes, which are situated on conjugative plasmids. All the genes encode small, toxic proteins of approximately 50 amino acids which are functionally and structurally homologous. Furthermore, the gene family shares post-transcriptional regulation of expression, albeit by different mechanisms. We demonstrate here that translation of gef is coupled to an upstream open reading frame which, in turn, is regulated by a transacting factor, probably an antisense RNA.
Original languageEnglish
JournalMolecular Microbiology
Volume5
Issue number7
Pages (from-to)1639-1648
ISSN0950-382X
DOIs
Publication statusPublished - 1991
Externally publishedYes

Cite this

Poulsen, Lars K. ; Refn, Annette ; Molin, Søren ; Andersson, Poul. / The gef gene from Escherichia coli is regulated at the level of translation. In: Molecular Microbiology. 1991 ; Vol. 5, No. 7. pp. 1639-1648.
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author = "Poulsen, {Lars K.} and Annette Refn and S{\o}ren Molin and Poul Andersson",
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The gef gene from Escherichia coli is regulated at the level of translation. / Poulsen, Lars K.; Refn, Annette; Molin, Søren; Andersson, Poul.

In: Molecular Microbiology, Vol. 5, No. 7, 1991, p. 1639-1648.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The gef gene from Escherichia coli is regulated at the level of translation

AU - Poulsen, Lars K.

AU - Refn, Annette

AU - Molin, Søren

AU - Andersson, Poul

PY - 1991

Y1 - 1991

N2 - We describe post-transcriptional regulation of the chromosomal gene, gef, from Escherichia coli. The gef gene is a member of a gene family consisting of the chromosomal gef and reIF genes from Escherichia coli and the hok, flmA, srnB, and pndA genes, which are situated on conjugative plasmids. All the genes encode small, toxic proteins of approximately 50 amino acids which are functionally and structurally homologous. Furthermore, the gene family shares post-transcriptional regulation of expression, albeit by different mechanisms. We demonstrate here that translation of gef is coupled to an upstream open reading frame which, in turn, is regulated by a transacting factor, probably an antisense RNA.

AB - We describe post-transcriptional regulation of the chromosomal gene, gef, from Escherichia coli. The gef gene is a member of a gene family consisting of the chromosomal gef and reIF genes from Escherichia coli and the hok, flmA, srnB, and pndA genes, which are situated on conjugative plasmids. All the genes encode small, toxic proteins of approximately 50 amino acids which are functionally and structurally homologous. Furthermore, the gene family shares post-transcriptional regulation of expression, albeit by different mechanisms. We demonstrate here that translation of gef is coupled to an upstream open reading frame which, in turn, is regulated by a transacting factor, probably an antisense RNA.

U2 - 10.1111/j.1365-2958.1991.tb01911.x

DO - 10.1111/j.1365-2958.1991.tb01911.x

M3 - Journal article

VL - 5

SP - 1639

EP - 1648

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 7

ER -