The flame retardant DE-71 (a mixture of polybrominated diphenyl ethers) inhibits human differentiated thyroid cell function in vitro

Thit Mynster Kronborg, Juliana Frohnert Hansen, Åse Krogh Rasmussen, Katrin Vorkamp, Claus Henrik Nielsen, Marie Frederiksen, Jacob Hofman-Bang, Christoffer Holst Hahn, Louise Ramhøj, Ulla Feldt-Rasmussen

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Abstract

Normal thyroid function is essential for general growth and metabolism, but can be affected by endocrine disrupting chemicals (EDCs). Polybrominated diphenyl ethers (PBDEs) have been used worldwide to reduce flammability in different materials and are suspected to be EDCs. The production of the commercial Penta- and OctaBDE mixtures is banned, but DecaBDEs and existing products may leak PBDEs into the environment. Our aim was to investigate the effect of the PentaBDE mixture DE-71 on human thyroid cells in vitro. Primary human thyroid cells were obtained as paraadenomatous tissue and cultured in monolayers. The influence of DE-71 on cyclic adenosine monophosphate (cAMP) and thyroglobulin (Tg) production was examined in the culture medium by competitive radioimmunoassay and enzyme-linked immunosorbent assay, respectively. Real-time quantitative PCR analysis of thyroid-specific genes was performed on the exposed cell cultures. PBDE concentrations were determined in cellular and supernatant fractions of the cultures. DE-71 inhibited Tg-release from TSH-stimulated thyrocytes. At 50 mg/L DE-71, mean Tg production was reduced by 71.9% (range: 8.5-98.7%), and cAMP by 95.1% (range: 91.5-98.8%) compared to controls). Expression of mRNA encoding Tg, TPO and TSHr were significantly inhibited (p
Original languageEnglish
Article numbere0179858
JournalP L o S One
Volume12
Issue number6
Number of pages18
ISSN1932-6203
DOIs
Publication statusPublished - 2017

Cite this

Kronborg, T. M., Hansen, J. F., Rasmussen, Å. K., Vorkamp, K., Nielsen, C. H., Frederiksen, M., Hofman-Bang, J., Hahn, C. H., Ramhøj, L., & Feldt-Rasmussen, U. (2017). The flame retardant DE-71 (a mixture of polybrominated diphenyl ethers) inhibits human differentiated thyroid cell function in vitro. P L o S One, 12(6), [e0179858]. https://doi.org/10.1371/journal.pone.0179858