The extracellular polysaccharide of Pichia (Hansenula) holstii NRRL Y-2448: The structure of the phosphomannan backbone

Lesley A.S. Parolis, Jens Øllgaard Duus, Haralambos Parolis, Morten Meldal, Klaus Bock

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The exopolysaccharide produced by Pichia (Hansenula) holstii NRRL Y-2448 is composed of a phosphomannan core to which oligosaccharide diester phosphate side chains are appended. The oligosaccharides of the side chains were released as oligosaccharide phosphates and neutral oligosaccharides by mild hydrolysis with aqueous acetic acid and aqueous hydrogen fluoride, respectively. The liberated oligosaccharide phosphates were studied by NMR spectroscopy and by electrospray and fast atom bombardment mass spectrometry. The structures of the neutral oligosaccharides were determined by 1D and 2D NMR spectroscopic experiments. Further insight into the length of the side chains was obtained from a matrix assisted laser desorption ionisation-time of flight mass spectrometric study of high and low molecular weight fragments obtained from partial acid hydrolysis of the native polysaccharide. © 1998 Elsevier Science Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Research
Volume293
Pages (from-to)101-117
ISSN0008-6215
DOIs
Publication statusPublished - 1996
Externally publishedYes

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