The efficiency of functional mitochondrial replacement in Saccharomyces species has directional character

P. Sulo, M. Spirek, A. Soltesova, G. Marinoni, Jure Piskur

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Optimal interactions among nuclear and mitochondria-coded proteins are required to assemble functional complexes of mitochondrial oxidative phosphorylation. The communication between the nuclear and mitochondrial genomes has been studied by transplacement of mitochondria from related species into mutants devoid of mitochondrial DNA (rho(0)). Recently we have reported that the mitochondria transferred from Saccharomyces paradoxus restored partially the respiration in Saccharomyces cerevisiae rho(0) mutants. Here we present evidence that the S. cerevisiae mitochondria completely salvage from respiration deficiency, not only in conspecific isolates but also in S. paradoxus. The respiratory capacity in less-related species can be recovered exclusively in the presence of S. cerevisiae chromosomes. The efficiency of the re-established oxidative phosphorylation did not rely on the presence of introns in the S. cerevisiae mitochondrial DNA. Our results suggest that, apart from evolutionary distance, the direction of mitochondrial replacement could play a significant role in installing the complete (wild-type-like) interaction between mitochondria and nuclei from different species.
    Original languageEnglish
    JournalF E M S Yeast Research
    Volume4
    Issue number1
    Pages (from-to)97-104
    ISSN1567-1356
    Publication statusPublished - 2003

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