The Effects of Temperature and Innate Immunity on Transmission of Campylobacter jejuni (Campylobacterales: Campylobacteraceae) Between Life Stages of Musca domestica (Diptera: Muscidae)

Simon Bahrndorff, C. Gill, C. Lowenberger, H. Skovgard, Birthe Hald

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The house fly (Musca domestica L.) is a well-established vector of human pathogens, including Campylobacter spp., which can cause infection of broiler chicken flocks, and through contaminated broiler meat can cause outbreaks of campylobacteriosis in humans. We investigated whether Campylobacter jejuni (Jones) could be transferred between life stages of M. domestica (larvae-pupae-adults) and determined bacterial counts of C. jejuni at different time points after bacterial exposure. C. jejuni was transmitted from infected larvae to pupae, but not to the adult stage. Infected larvae maintained at 25 degrees C had mean bacterial numbers of 6.5 +/- 0.2 SE log(10) (colony forming units [CFU]/g) that subsequently dropped to 3.6 +/- 0.3 SE log(10) (CFU/g) 8 h after infection. Pupae originating from infected larvae contained mean bacterial numbers of 5.3 +/- 0.1 SE log(10) (CFU/g), and these numbers dropped to 4.8 +/- 0.1 SE log(10) (CFU/g) 24 h after pupation. The decline in C. jejuni numbers during pupal development coincided with increased expression of antimicrobial peptides, including cecropin, diptericin, attacin, and defensin, in the larva-pupa transition stage and a later second peak in older pupae (4 or 48 h). Conversely, there was a reduced expression of the digestive enzyme, lysozyme, in pupae and adults compared with larvae.
Original languageEnglish
JournalJournal of Medical Entomology
Volume51
Issue number3
Pages (from-to)670-677
Number of pages8
ISSN0022-2585
DOIs
Publication statusPublished - 2014

Keywords

  • ENTOMOLOGY
  • VETERINARY
  • HOUSE-FLY DIPTERA
  • RHODNIUS-PROLIXUS
  • ANTIMICROBIAL PEPTIDE
  • ESCHERICHIA-COLI
  • INSECT DEFENSIN
  • MOLECULAR CHARACTERIZATION
  • DIGESTIVE FUNCTION
  • BROILER-CHICKENS
  • CHAGAS-DISEASE
  • AEDES-AEGYPTI
  • antimicrobial peptide
  • infection
  • vector
  • campylobacteriosis
  • house fly

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