The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type

Hans-Christian Ingerslev, L. von Gersdorff Jørgensen, Mikael Lenz Strube, N. Larsen, Inger Dalsgaard, Mette Boye, Lone Madsen

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhynchus mykiss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding to seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediminibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards phylum Firmicutes dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After first-feeding, there were significantly higher abundances of Streptococcus, Leuconostoc and Weissella in fish fed the plant-based diet. The microbiota clustered separately according to the diet type, but only minor effects were seen from the probiont when using PCA-analysis. The constitutive transcription level of most examined immune genes increased during the ontogenic shift, but the results could not explain the differences in the composition of the microbiota dependent on diet treatment after first-feeding. The results suggest that the intestine of rainbow trout is colonised at an early state, but is guided in new and different directions dependent on the diet type.
    Original languageEnglish
    JournalAquaculture
    Volume424–425
    Pages (from-to)24–34
    ISSN0044-8486
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Ontogeny
    • First-feeding
    • Microbiota
    • Rainbow trout
    • 16S rRNA
    • Probiotics

    Cite this

    Ingerslev, Hans-Christian ; von Gersdorff Jørgensen, L. ; Strube, Mikael Lenz ; Larsen, N. ; Dalsgaard, Inger ; Boye, Mette ; Madsen, Lone. / The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type. In: Aquaculture. 2014 ; Vol. 424–425. pp. 24–34.
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    abstract = "An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhynchus mykiss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding to seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediminibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards phylum Firmicutes dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After first-feeding, there were significantly higher abundances of Streptococcus, Leuconostoc and Weissella in fish fed the plant-based diet. The microbiota clustered separately according to the diet type, but only minor effects were seen from the probiont when using PCA-analysis. The constitutive transcription level of most examined immune genes increased during the ontogenic shift, but the results could not explain the differences in the composition of the microbiota dependent on diet treatment after first-feeding. The results suggest that the intestine of rainbow trout is colonised at an early state, but is guided in new and different directions dependent on the diet type.",
    keywords = "Ontogeny, First-feeding, Microbiota, Rainbow trout, 16S rRNA, Probiotics",
    author = "Hans-Christian Ingerslev and {von Gersdorff J{\o}rgensen}, L. and Strube, {Mikael Lenz} and N. Larsen and Inger Dalsgaard and Mette Boye and Lone Madsen",
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    The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type. / Ingerslev, Hans-Christian; von Gersdorff Jørgensen, L.; Strube, Mikael Lenz; Larsen, N.; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.

    In: Aquaculture, Vol. 424–425, 2014, p. 24–34.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type

    AU - Ingerslev, Hans-Christian

    AU - von Gersdorff Jørgensen, L.

    AU - Strube, Mikael Lenz

    AU - Larsen, N.

    AU - Dalsgaard, Inger

    AU - Boye, Mette

    AU - Madsen, Lone

    PY - 2014

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    N2 - An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhynchus mykiss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding to seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediminibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards phylum Firmicutes dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After first-feeding, there were significantly higher abundances of Streptococcus, Leuconostoc and Weissella in fish fed the plant-based diet. The microbiota clustered separately according to the diet type, but only minor effects were seen from the probiont when using PCA-analysis. The constitutive transcription level of most examined immune genes increased during the ontogenic shift, but the results could not explain the differences in the composition of the microbiota dependent on diet treatment after first-feeding. The results suggest that the intestine of rainbow trout is colonised at an early state, but is guided in new and different directions dependent on the diet type.

    AB - An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhynchus mykiss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding to seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediminibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards phylum Firmicutes dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After first-feeding, there were significantly higher abundances of Streptococcus, Leuconostoc and Weissella in fish fed the plant-based diet. The microbiota clustered separately according to the diet type, but only minor effects were seen from the probiont when using PCA-analysis. The constitutive transcription level of most examined immune genes increased during the ontogenic shift, but the results could not explain the differences in the composition of the microbiota dependent on diet treatment after first-feeding. The results suggest that the intestine of rainbow trout is colonised at an early state, but is guided in new and different directions dependent on the diet type.

    KW - Ontogeny

    KW - First-feeding

    KW - Microbiota

    KW - Rainbow trout

    KW - 16S rRNA

    KW - Probiotics

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    DO - 10.1016/j.aquaculture.2013.12.032

    M3 - Journal article

    VL - 424–425

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    JO - Aquaculture

    JF - Aquaculture

    SN - 0044-8486

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