The Determination of Polyethylene Glycol in Untreated Urine Samples by High Performance Liquid Chromatography for Intestinal Permeability Studies

Elfinn Larsen, Walther Batsberg Pedersen, E. Philipsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Polyethylene glycol in urine samples has been investigated by high performance liquid chromatography. The molecular weights ranged from 634 to 1338. The urine samples were applied to the chromatographic system without any pre-treatment. For samples with a concentration of 0.2% polyethylene glycol a relative standard deviation of 2% was obtained at best. Twenty-six volunteers ingested ten grams of polyethylene glycol and the amount excreted in the urine during the first 6 hours was measured. The recoveries of the individual oligomers were found to be in accordance with those previously published where an extraction procedure was included. The mean recovery was 12.2 .+-. 0.08% for polyethylene glycol with MW = 634 decreasing to 2.1 .+-. 0.3% for MW = 1206.
    Original languageEnglish
    JournalJournal of Trace and Microprobe Techniques
    Volume3
    Issue number3
    Pages (from-to)255-271
    ISSN0733-4680
    Publication statusPublished - 1985

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