The CytR repressor antagonizes cAMP CRP activation in the deoP2 promoter of Escherichia coli.

Lotte Søgaard-Andersen, Jan Martinussen, Niels E. Møllegaard, Stephen Douthwaite, Poul Valentin-Hansen

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Abstract

We have investigated the regulation of the Escherichia coli deoCp2 promoter by the CytR repressor and the cyclic AMP (cAMP) receptor protein (CRP) complexed to cAMP. Promoter regions controlled by these two proteins characteristically contain tandem cAMP-CRP binding sites. Here we show that (i) CytR selectively
regulated cAMP-CRP-dependent initiations, although transcription started from the same site in deoCp2 in the absence or presence of cAMP-CRP; (ii) deletion of the uppermost cAMP-CRP target (CRP-2) resulted in loss of CytR regulation, but had only a minor effect on positive control by the cAMP-CRP complex; (iui) introduction of point mutations in either CRP target resulted in loss of CytR regulation; and (iv) regulation by CytR of deletion mutants lacking CRP-2 could be specifically reestablished by increasing the intracellular concentration of CytR. These findings indicate that both CRP targets are required for efficient CytR repression of deoCp2. Models for the action of CytR are discussed in light of these findings.
Original languageEnglish
JournalJournal of Bacteriology
Volume172
Issue number10
Pages (from-to)5706-5713
ISSN0021-9193
DOIs
Publication statusPublished - 1990
Externally publishedYes

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