The chromosome complement of Erysiphe graminis f.sp. Hordei analysed by light microscopy and field inversion gel electrophoresis

L. Borbye; I. Linde-Laursen; S.K. Christiansen; H. Giese

doi:10.1016/S0953-7562(09)80922-2

The chromosome complement of Erysiphe graminis f.sp. Hordei analysed by light microscopy and field inversion gel electrophoresis

L. Borbye
, I. Linde-Laursen
, S.K. Christiansen
, H. Giese

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0·2–0·8 Mb) and a group of at least two larger chromosomes.

Original language	English
Journal	Mycological Research
Volume	96
Issue number	2
Pages (from-to)	97-102
ISSN	1878-6146
DOIs	https://doi.org/10.1016/S0953-7562(09)80922-2
Publication status	Published - 1992

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title = "The chromosome complement of Erysiphe graminis f.sp. Hordei analysed by light microscopy and field inversion gel electrophoresis",

abstract = "Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0·2–0·8 Mb) and a group of at least two larger chromosomes.",

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author = "L. Borbye and I. Linde-Laursen and S.K. Christiansen and H. Giese",

year = "1992",

doi = "10.1016/S0953-7562(09)80922-2",

language = "English",

volume = "96",

pages = "97--102",

journal = "Mycological Research",

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T1 - The chromosome complement of Erysiphe graminis f.sp. Hordei analysed by light microscopy and field inversion gel electrophoresis

AU - Borbye, L.

AU - Linde-Laursen, I.

AU - Christiansen, S.K.

AU - Giese, H.

PY - 1992

Y1 - 1992

N2 - Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0·2–0·8 Mb) and a group of at least two larger chromosomes.

AB - Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0·2–0·8 Mb) and a group of at least two larger chromosomes.

KW - Begrænsning af miljøbelastninger

U2 - 10.1016/S0953-7562(09)80922-2

DO - 10.1016/S0953-7562(09)80922-2

M3 - Journal article

SN - 1878-6146

VL - 96

SP - 97

EP - 102

JO - Mycological Research

JF - Mycological Research

IS - 2

ER -

The chromosome complement of Erysiphe graminis f.sp. Hordei analysed by light microscopy and field inversion gel electrophoresis

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