The cda GenoTox assay: A new and sensitive method for detection of environmental genotoxins, including nitroarenes and aromatic amines

Trine G. Østergaard, Lars Henrik Hansen, Mona-Lise Binderup, Anders Norman, Søren J. Sørensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A new bacterial test system for detection of genotoxic compounds was developed, based on two new Sahnonella typhimurium tester strains, TGO1 and TGO2. Both strains contain a gene fusion between a strong SOS-promotor, P-cda, and the gfp gene, which allows detection of genotoxic compounds that induce the SOS response. SOS induction was detected by means of flow cytometry. TGO1 showed an increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine compared with a previously developed strain, which had an Escherichia coli strain as host instead of S. typhimurium. S9 mix was introduced into the assay, making the test system suitable for detection of indirect mutagens. Furthermore, the genes for bacterial nitro-reductase (NR) and o-acetyl transferase (o-AT) were inserted into TGO2, making it an NR- and o-AT-over-expressing strain. This resulted in an assay that was able to detect the nitroarene I -nitropyrene and the aromatic amine 2-aminoanthracene with high sensitivity.
Original languageEnglish
JournalMutation Research-genetic Toxicology and Environmental Mutagenesis
Volume631
Issue number2
Pages (from-to)77-84
ISSN1383-5718
DOIs
Publication statusPublished - 2007

Keywords

  • strains
  • environmental genotoxins
  • bacterial tests

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