The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically

    Research output: Contribution to journalJournal articlepeer-review

    Abstract

    The biosynthesis of carbamoylphosphate is catalysed by the heterodimeric enzyme carbamoylphosphate synthetase (CPSase). The genes encoding the two subunits in procaryotes are normally transcribed as an operon, whereas in Lactococcus lactis, the gene encoding the large subunit (carB) is shown to be an isolated transcriptional unit. Carbamoylphosphate is a precursor in the biosynthesis of both pyrimidine nucleotides and arginine. By mutant analysis L. lactis is shown to possess only one carB gene; the same gene product is thus required for both biosynthetic pathways. Furthermore, arginine may satisfy the requirement for carbamoylphosphate in pyrimidine biosynthesis through degradation by the arginine deiminase pathway. The expression of the carB gene is subject to regulation at the level of transcription by pyrimidines most probably by an attenuator mechanism. Upstream of the carB gene, an open reading frame, showing a high degree of similarity to glutathione peroxidases from other organisms was identified.
    Original languageEnglish
    JournalJournal of Bacteriology
    Volume180
    Issue number17
    Pages (from-to)4380-4386
    Number of pages7
    ISSN0021-9193
    DOIs
    Publication statusPublished - 1998

    Keywords

    • Science/technology
    • Amino Acid Sequence
    • Base Sequence
    • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
    • Chromosomes, Bacterial
    • Cloning, Molecular
    • DNA, Bacterial
    • Gene Expression Regulation, Bacterial
    • Gene Expression Regulation, Enzymologic
    • Genes, Bacterial
    • Lactococcus lactis
    • Mutation
    • Pyrimidines
    • Transcription, Genetic
    • EC 6.3.5.5 Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
    • carbamoyl phosphate
    • carbamoyl phosphate synthase
    • glutathione peroxidase
    • allele
    • article
    • cistron
    • cloning
    • DNA sequence
    • enzyme assay
    • enzyme synthesis
    • gene expression regulation
    • genetic transcription
    • nonhuman
    • nucleotide sequence
    • operon
    • plasmid
    • priority journal
    • pyrimidine synthesis
    • Posibacteria
    • Prokaryota
    • Streptococcus
    • MICROBIOLOGY
    • SUBTILIS PYR OPERON
    • TRANSCRIPTIONAL ATTENUATION
    • SUBSP CREMORIS
    • PYRIMIDINE BIOSYNTHESIS
    • STREPTOCOCCUS-LACTIS
    • REGULATORY PROTEIN
    • MESSENGER-RNA
    • IDENTIFICATION
    • EXPRESSION
    • METABOLISM
    • monocistronic gene transcription
    • transcription
    • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Lactococcus-lactis
    • carB gene
    • carbamoylphosphate 590-55-6 synthesis
    • carbamoylphosphate synthetase 9026-23-7Q, 37233-48-0Q, 81295-60-5Q large subunit gene
    • enzymes
    • pyrimidine nucleotides synthesis
    • 10010, Comparative biochemistry
    • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
    • 10064, Biochemistry studies - Proteins, peptides and amino acids
    • 10300, Replication, transcription, translation
    • 10506, Biophysics - Molecular properties and macromolecules
    • 10804, Enzymes - Methods
    • 13002, Metabolism - General metabolism and metabolic pathways
    • 31000, Physiology and biochemistry of bacteria
    • 31500, Genetics of bacteria and viruses
    • Biochemistry and Molecular Biophysics
    • AJ000109 EMBL nucleotide sequence
    • Enzymology
    • Molecular Genetics

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