The Botrytis cinerea type III polyketide synthase shows unprecedented high catalytic efficiency toward long chain acyl-CoAs

Marimuthu Jeya, Tae-Su Kim, Manish Kumar Tiwari, Jinglin Li, Huimin Zhao, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

BPKS from Botrytis cinerea is a novel type III polyketide synthase that accepts C-4-C-18 aliphatic acyl-CoAs and benzoyl-CoA as the starters to form pyrones, resorcylic acids and resorcinols through sequential condensation with malonyl-CoA. The catalytic efficiency (k(cat)/K-m) of BPKS was 2.8 x 10(5) s(-1) M-1 for palmitoyl-CoA, the highest ever reported. Substrate docking analyses addressed the unique features of BPKS such as its high activity and high specificity toward long chain acyl-CoAs.
Original languageEnglish
JournalMolecular Biosystems
Volume8
Issue number11
Pages (from-to)2864-2867
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Acyl Coenzyme A
  • Botrytis
  • Catalysis
  • Fungal Proteins
  • Polyketide Synthases
  • Substrate Specificity
  • 79956-01-7 Polyketide Synthases
  • acyl coenzyme A
  • fungal protein
  • polyketide synthase
  • article
  • catalysis
  • enzyme specificity
  • enzymology
  • metabolism
  • high activity
  • high catalytic efficiency
  • high specificity
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Botrytis cinerea species
  • acyl-CoA
  • benzoyl-CoA 6756-74-7
  • pyrone 96345-33-4
  • resorcinols
  • resorcylic acid 27138-57-4
  • type III polyketide synthase
  • 03504, Genetics - Plant
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 51518, Plant physiology - Enzymes
  • Biochemistry and Molecular Biophysics
  • XM001555277.1 GenBank, EMBL, DDJB nucleotide sequence
  • Enzymology
  • BIOCHEMISTRY
  • CHALCONE SYNTHASE
  • ASPERGILLUS-ORYZAE
  • NEUROSPORA-CRASSA
  • BIOSYNTHESIS
  • SUPERFAMILY
  • MECHANISMS
  • DISCOVERY

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