The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.
Original languageEnglish
JournalEpidemiology and Infection
Volume145
Issue number13
Pages (from-to)2827-2837
Number of pages11
ISSN0950-2688
DOIs
Publication statusPublished - 2017

Keywords

  • Antimicrobial drugs
  • antimicrobial resistance in agricultural settings
  • cultivation
  • metagenomic
  • pigs

Cite this

@article{5ec26f7c468849f2823df6f0032dff56,
title = "The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches",
abstract = "The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.",
keywords = "Antimicrobial drugs, antimicrobial resistance in agricultural settings, cultivation, metagenomic, pigs",
author = "{Dalhoff Andersen}, Vibe and {de Knegt}, Leonardo and Patrick Munk and Jensen, {Marie Stengaard} and Yvonne Agers{\o} and Aarestrup, {Frank M{\o}ller} and H{\aa}kan Vigre",
year = "2017",
doi = "10.1017/S0950268817001285",
language = "English",
volume = "145",
pages = "2827--2837",
journal = "Epidemiology and Infection",
issn = "0950-2688",
publisher = "Cambridge University Press",
number = "13",

}

The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches. / Dalhoff Andersen, Vibe; de Knegt, Leonardo; Munk, Patrick; Jensen, Marie Stengaard; Agersø, Yvonne; Aarestrup, Frank Møller; Vigre, Håkan.

In: Epidemiology and Infection, Vol. 145, No. 13, 2017, p. 2827-2837.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches

AU - Dalhoff Andersen, Vibe

AU - de Knegt, Leonardo

AU - Munk, Patrick

AU - Jensen, Marie Stengaard

AU - Agersø, Yvonne

AU - Aarestrup, Frank Møller

AU - Vigre, Håkan

PY - 2017

Y1 - 2017

N2 - The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.

AB - The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.

KW - Antimicrobial drugs

KW - antimicrobial resistance in agricultural settings

KW - cultivation

KW - metagenomic

KW - pigs

U2 - 10.1017/S0950268817001285

DO - 10.1017/S0950268817001285

M3 - Journal article

VL - 145

SP - 2827

EP - 2837

JO - Epidemiology and Infection

JF - Epidemiology and Infection

SN - 0950-2688

IS - 13

ER -