Abstract
The usefulness of enzyme-linked immunosorbent assay (ELISA) was investigated as a simple method to screen for Salmonella Dublin infection in dairy herds, examining bulk tank milk samples for lipopolysaccharide (O:1,9,12) antibodies. The cut-off value for the ELISA on bulk tank milk was established based on individual milk samples (n = 2887) and bulk tank milk from 52 herds. Bulk tank milk samples (n = 5108) were collected from 1464 dairy herds located in 19 different areas. About 10% of the dairy herds in Denmark participated in the study. The percentage of herds changing from test-negative to test-positive in each area was correlated with the incidence of S. Dublin outbreaks in the corresponding county (r = 0.48, n = 19; P <0.025). The mean level of the OD values obtained in the first and third test rounds was not constant (Pr \t\ = 0.0001). The study demonstrated that the probability of being test-negative in the third test round was 0.926 for a herd with 2 previous test-negative results. It was concluded that the investigated ELISA method was in general accordance with the cases of clinical S. Dublin infection recorded. and that the method has a potential for national screening purposes.
| Original language | English |
|---|---|
| Journal | Canadian Journal of Veterinary Research |
| Volume | 65 |
| Issue number | 1 |
| Pages (from-to) | 15-21 |
| ISSN | 0830-9000 |
| Publication status | Published - 2001 |
Cite this
Wedderkopp, A., Stroger, U., Bitsch, V., & Lind, P. (2001). Testing of bulk tank milk for Salmonella Dublin infection in Danish dairy herds. Canadian Journal of Veterinary Research, 65(1), 15-21.