Terverticillate penicillia studied by direct electrospray mass spectrometric profiling of crude extracts II. Database and identification

Jørn Smedsgaard

doi:10.1016/S0305-1978(96)00087-7

Terverticillate penicillia studied by direct electrospray mass spectrometric profiling of crude extracts II. Database and identification

Jørn Smedsgaard

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A mass spectral database was built using standard instrument software from 678 electrospray mass spectra (mass profiles) from crude fungal extracts of terverticillate taxa within the genus Penicillium. The match factors calculated from searching all the mass profiles stored in the database were used as a distance measure for grouping into taxa. Isolates from more than 75% of the taxa were placed correctly, that is with the highest match factors to other isolates of the their own species by library searches. The database was used as an expert system based on correct identification of the isolates stored according to classical taxonomic criteria. Mass profiles collected in previous studies could be identified by a search in the database. (C) 1997 Elsevier Science Ltd.

Original language	English
Journal	Biochemical Systematics and Ecology
Volume	25
Pages (from-to)	65-71
ISSN	0305-1978
DOIs	https://doi.org/10.1016/S0305-1978(96)00087-7
Publication status	Published - 1997

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abstract = "A mass spectral database was built using standard instrument software from 678 electrospray mass spectra (mass profiles) from crude fungal extracts of terverticillate taxa within the genus Penicillium. The match factors calculated from searching all the mass profiles stored in the database were used as a distance measure for grouping into taxa. Isolates from more than 75% of the taxa were placed correctly, that is with the highest match factors to other isolates of the their own species by library searches. The database was used as an expert system based on correct identification of the isolates stored according to classical taxonomic criteria. Mass profiles collected in previous studies could be identified by a search in the database. (C) 1997 Elsevier Science Ltd. ",

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AU - Smedsgaard, Jørn

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AB - A mass spectral database was built using standard instrument software from 678 electrospray mass spectra (mass profiles) from crude fungal extracts of terverticillate taxa within the genus Penicillium. The match factors calculated from searching all the mass profiles stored in the database were used as a distance measure for grouping into taxa. Isolates from more than 75% of the taxa were placed correctly, that is with the highest match factors to other isolates of the their own species by library searches. The database was used as an expert system based on correct identification of the isolates stored according to classical taxonomic criteria. Mass profiles collected in previous studies could be identified by a search in the database. (C) 1997 Elsevier Science Ltd.

U2 - 10.1016/S0305-1978(96)00087-7

DO - 10.1016/S0305-1978(96)00087-7

M3 - Journal article

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VL - 25

SP - 65

EP - 71

JO - Biochemical Systematics and Ecology

JF - Biochemical Systematics and Ecology

ER -

Terverticillate penicillia studied by direct electrospray mass spectrometric profiling of crude extracts II. Database and identification

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