Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells

Seyed Mohammad Amin Haramshahi, Shahin Bonakdar, Mehdi Moghtadaei, Khorshid Kamguyan, Esben Thormann, Sara Tanbakooei, Sara Simorgh, Peiman Brouki Milan, Naser Amini, Noor Ahmad Latifi, Mohammad Taghi Joghataei, Ali Samadikuchaksaraei, Majid Katebi, Mansoureh Soleimani*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Tendon tissue engineering based on stem cell differentiation has attracted a great deal of attention in recent years. Previous studies have examined the effect of cell-imprinted polydimethylsiloxane (PDMS) substrate on induction differentiation in stem cells. In this study, we used tenocyte morphology as a positive mold to create a tenocyte-imprinted substrate on PDMS. The morphology and topography of this tenocyte replica on PDMS was evaluated with scanning electron microscopy and atomic force microscopy. Then the tenogenic differentiation induction capacity of tenocytes replica in ADSCs was investigated and compared with other groups including tissue replica (Which was produced similar to the tenocyte replica and was evaluated by SEM), decellularized tendon, and BMP-12 as other potentially inducers. This comparison gives us an estimate of the ability of tenocyte-imprinted PDMS (which called cell replica in the present study) to induce differentiation compared to other inducers. For this reason ADSCs were divided into 5 groups including control, cell replica, tissue replica, decellularized tendon and BMP-12. ADSCs were seeded on each group seperately and investigated by real-time RT-PCR after 7 and 14 days. Our results showed that in spite of the higher effect of growth factor on tenogenic differentiation, cell replica can also induce tenocyte marker expression (Scleraxis and Tenomodulin) in ADSCs. Moreover, tenogenic differentiation induction capacity of cell replica was greater than tissue replica. Immunocytochemistry analysis revealed that ADSCs seeding on cell replica for 14 days led to Scleraxis and Tenomodulin expression at the protein level. Also, immunohistochemistry indicated that contrary to the promising results in vitro, there was little difference between ADSCs cultured on tenocyte-imprinted PDMS and untreated ADSCs. The results of such studies could lead to the production of inexpensive cell culture plates or biomaterials that can induce differentiation in stem cells without growth factors or other supplements.
Original languageEnglish
JournalBiomedical Materials (Bristol)
ISSN1748-6041
DOIs
Publication statusAccepted/In press - 2020

Cite this

Haramshahi, Seyed Mohammad Amin ; Bonakdar, Shahin ; Moghtadaei, Mehdi ; Kamguyan, Khorshid ; Thormann, Esben ; Tanbakooei, Sara ; Simorgh, Sara ; Milan, Peiman Brouki ; Amini, Naser ; Latifi, Noor Ahmad ; Joghataei, Mohammad Taghi ; Samadikuchaksaraei, Ali ; Katebi, Majid ; Soleimani, Mansoureh. / Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells. In: Biomedical Materials (Bristol). 2020.
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title = "Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells",
abstract = "Tendon tissue engineering based on stem cell differentiation has attracted a great deal of attention in recent years. Previous studies have examined the effect of cell-imprinted polydimethylsiloxane (PDMS) substrate on induction differentiation in stem cells. In this study, we used tenocyte morphology as a positive mold to create a tenocyte-imprinted substrate on PDMS. The morphology and topography of this tenocyte replica on PDMS was evaluated with scanning electron microscopy and atomic force microscopy. Then the tenogenic differentiation induction capacity of tenocytes replica in ADSCs was investigated and compared with other groups including tissue replica (Which was produced similar to the tenocyte replica and was evaluated by SEM), decellularized tendon, and BMP-12 as other potentially inducers. This comparison gives us an estimate of the ability of tenocyte-imprinted PDMS (which called cell replica in the present study) to induce differentiation compared to other inducers. For this reason ADSCs were divided into 5 groups including control, cell replica, tissue replica, decellularized tendon and BMP-12. ADSCs were seeded on each group seperately and investigated by real-time RT-PCR after 7 and 14 days. Our results showed that in spite of the higher effect of growth factor on tenogenic differentiation, cell replica can also induce tenocyte marker expression (Scleraxis and Tenomodulin) in ADSCs. Moreover, tenogenic differentiation induction capacity of cell replica was greater than tissue replica. Immunocytochemistry analysis revealed that ADSCs seeding on cell replica for 14 days led to Scleraxis and Tenomodulin expression at the protein level. Also, immunohistochemistry indicated that contrary to the promising results in vitro, there was little difference between ADSCs cultured on tenocyte-imprinted PDMS and untreated ADSCs. The results of such studies could lead to the production of inexpensive cell culture plates or biomaterials that can induce differentiation in stem cells without growth factors or other supplements.",
author = "Haramshahi, {Seyed Mohammad Amin} and Shahin Bonakdar and Mehdi Moghtadaei and Khorshid Kamguyan and Esben Thormann and Sara Tanbakooei and Sara Simorgh and Milan, {Peiman Brouki} and Naser Amini and Latifi, {Noor Ahmad} and Joghataei, {Mohammad Taghi} and Ali Samadikuchaksaraei and Majid Katebi and Mansoureh Soleimani",
year = "2020",
doi = "10.1088/1748-605x/ab6709",
language = "English",
journal = "Biomedical Materials (Bristol)",
issn = "1748-6041",
publisher = "IOP Publishing",

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Haramshahi, SMA, Bonakdar, S, Moghtadaei, M, Kamguyan, K, Thormann, E, Tanbakooei, S, Simorgh, S, Milan, PB, Amini, N, Latifi, NA, Joghataei, MT, Samadikuchaksaraei, A, Katebi, M & Soleimani, M 2020, 'Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells', Biomedical Materials (Bristol). https://doi.org/10.1088/1748-605x/ab6709

Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells. / Haramshahi, Seyed Mohammad Amin; Bonakdar, Shahin; Moghtadaei, Mehdi; Kamguyan, Khorshid; Thormann, Esben; Tanbakooei, Sara; Simorgh, Sara; Milan, Peiman Brouki; Amini, Naser; Latifi, Noor Ahmad; Joghataei, Mohammad Taghi; Samadikuchaksaraei, Ali; Katebi, Majid; Soleimani, Mansoureh.

In: Biomedical Materials (Bristol), 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Tenocyte-imprinted substrate: a topography-based inducer for tenogenic differentiation in adipose tissue-derived mesenchymal stem cells

AU - Haramshahi, Seyed Mohammad Amin

AU - Bonakdar, Shahin

AU - Moghtadaei, Mehdi

AU - Kamguyan, Khorshid

AU - Thormann, Esben

AU - Tanbakooei, Sara

AU - Simorgh, Sara

AU - Milan, Peiman Brouki

AU - Amini, Naser

AU - Latifi, Noor Ahmad

AU - Joghataei, Mohammad Taghi

AU - Samadikuchaksaraei, Ali

AU - Katebi, Majid

AU - Soleimani, Mansoureh

PY - 2020

Y1 - 2020

N2 - Tendon tissue engineering based on stem cell differentiation has attracted a great deal of attention in recent years. Previous studies have examined the effect of cell-imprinted polydimethylsiloxane (PDMS) substrate on induction differentiation in stem cells. In this study, we used tenocyte morphology as a positive mold to create a tenocyte-imprinted substrate on PDMS. The morphology and topography of this tenocyte replica on PDMS was evaluated with scanning electron microscopy and atomic force microscopy. Then the tenogenic differentiation induction capacity of tenocytes replica in ADSCs was investigated and compared with other groups including tissue replica (Which was produced similar to the tenocyte replica and was evaluated by SEM), decellularized tendon, and BMP-12 as other potentially inducers. This comparison gives us an estimate of the ability of tenocyte-imprinted PDMS (which called cell replica in the present study) to induce differentiation compared to other inducers. For this reason ADSCs were divided into 5 groups including control, cell replica, tissue replica, decellularized tendon and BMP-12. ADSCs were seeded on each group seperately and investigated by real-time RT-PCR after 7 and 14 days. Our results showed that in spite of the higher effect of growth factor on tenogenic differentiation, cell replica can also induce tenocyte marker expression (Scleraxis and Tenomodulin) in ADSCs. Moreover, tenogenic differentiation induction capacity of cell replica was greater than tissue replica. Immunocytochemistry analysis revealed that ADSCs seeding on cell replica for 14 days led to Scleraxis and Tenomodulin expression at the protein level. Also, immunohistochemistry indicated that contrary to the promising results in vitro, there was little difference between ADSCs cultured on tenocyte-imprinted PDMS and untreated ADSCs. The results of such studies could lead to the production of inexpensive cell culture plates or biomaterials that can induce differentiation in stem cells without growth factors or other supplements.

AB - Tendon tissue engineering based on stem cell differentiation has attracted a great deal of attention in recent years. Previous studies have examined the effect of cell-imprinted polydimethylsiloxane (PDMS) substrate on induction differentiation in stem cells. In this study, we used tenocyte morphology as a positive mold to create a tenocyte-imprinted substrate on PDMS. The morphology and topography of this tenocyte replica on PDMS was evaluated with scanning electron microscopy and atomic force microscopy. Then the tenogenic differentiation induction capacity of tenocytes replica in ADSCs was investigated and compared with other groups including tissue replica (Which was produced similar to the tenocyte replica and was evaluated by SEM), decellularized tendon, and BMP-12 as other potentially inducers. This comparison gives us an estimate of the ability of tenocyte-imprinted PDMS (which called cell replica in the present study) to induce differentiation compared to other inducers. For this reason ADSCs were divided into 5 groups including control, cell replica, tissue replica, decellularized tendon and BMP-12. ADSCs were seeded on each group seperately and investigated by real-time RT-PCR after 7 and 14 days. Our results showed that in spite of the higher effect of growth factor on tenogenic differentiation, cell replica can also induce tenocyte marker expression (Scleraxis and Tenomodulin) in ADSCs. Moreover, tenogenic differentiation induction capacity of cell replica was greater than tissue replica. Immunocytochemistry analysis revealed that ADSCs seeding on cell replica for 14 days led to Scleraxis and Tenomodulin expression at the protein level. Also, immunohistochemistry indicated that contrary to the promising results in vitro, there was little difference between ADSCs cultured on tenocyte-imprinted PDMS and untreated ADSCs. The results of such studies could lead to the production of inexpensive cell culture plates or biomaterials that can induce differentiation in stem cells without growth factors or other supplements.

U2 - 10.1088/1748-605x/ab6709

DO - 10.1088/1748-605x/ab6709

M3 - Journal article

JO - Biomedical Materials (Bristol)

JF - Biomedical Materials (Bristol)

SN - 1748-6041

ER -