Taxonomic evidence that Vibrio carchariae Grimes et al. 1985 is a junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981

Karl Pedersen, Linda Verdonck, Brian Austin, Dawn A. Austin, Anicet R. Blanch, Patrick A. D. Grimont, Joan Jofre, Stéphania Koblavi, Jens Laurits Larsen, Tarja Tiainen, Martine Vigneulle, Jean Swings

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Abstract

A collection of 94 Vibrio isolates closely related to Vibrio harveyi, together with named reference and type strains, were investigated for phenotypic and genotypic properties. Using amplified fragment length polymorphism (AFLP), nine clusters were recognized. The largest cluster (n = 36), considered to be the bona fide V. harveyi group, contained the type strains of V. harveyi and Vibrio carchariae and most of the strains isolated from fish. The type strains of all other species, including Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio campbellii and Vibrio natriegens, clustered outside this group. By ribotyping, V. harveyi and V. carchariae patterns were very similar, insofar as they shared most bands. The V. campbellii type strain had several bands in common with the type strains of both V. harveyi and V. carchariae, whereas the other species were clearly distinct from these three species. DNA-DNA hybridization experiments showed 88% DNA binding between the type strains of V. harveyi and V. carchariae, whereas the DNA binding between V. harveyi and V. campbellii was 40%. Although the delineation of the species V. harveyi is still uncertain, the authors propose, on the basis of a number of tests, to delineate a core of V. harveyi strains which contained the type strains of both V. harveyi and V. carchariae. It is concluded that V. carchariae is the junior synonym of V. harveyi.
Original languageEnglish
JournalInternational Journal of Systematic Bacteriology
Volume48
Issue number3
Pages (from-to)749-758
Number of pages10
ISSN0020-7713
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • dna
  • article
  • bacterium isolation
  • dna dna hybridization
  • genotype
  • nonhuman
  • phenotype
  • priority journal
  • ribotyping
  • strain difference
  • vibrio
  • vibrio harveyi
  • Vibrio alginolyticus
  • Vibrio campbellii
  • Vibrio carchariae
  • Vibrio harveyi
  • Vibrio natriegens
  • Vibrio parahaemolyticus
  • AFLP
  • DNA-DNA hybridization
  • Ribotyping
  • Vibrio species
  • synonymy
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio-alginolyticus genetics, nomenclature, reclassification, taxonomy Vibrio-capmpbelli genetics, reclassification, taxonomy, nomenclature Vibrio-carchariae genetics, taxonomy, reclassification, nomenclature Vibrio-harveyi Vibrio-natriegens genetics, nomenclature, reclassification, taxonomy Vibrio-parahaemolyticus genetics, nomenclature, reclassification, taxonomy
  • 16S rRNA 16S ribosomal RNA
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 10010, Comparative biochemistry
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • DNA-DNA hybridization analytical method, molecular genetic method
  • ribotyping analytical method
  • Systematics and Taxonomy

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