Abstract
Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
| Original language | English |
|---|---|
| Title of host publication | NMR-Based Metabolomics |
| Editors | G. Gowda, D. Raftery |
| Publisher | Springer |
| Publication date | 2019 |
| Pages | 385-393 |
| Chapter | 21 |
| ISBN (Print) | 978-1-4939-9689-6 |
| DOIs | |
| Publication status | Published - 2019 |
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2037 |
| ISSN | 1064-3745 |
Keywords
- dDNP
- Hyperpolarization
- 13C-NMR
- Glucose
Cite this
Lerche, M. H., Karlsson, M., Ardenkjær-Larsen, J. H., & Jensen, P. R. (2019). Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. In G. Gowda, & D. Raftery (Eds.), NMR-Based Metabolomics (pp. 385-393). Springer. Methods in Molecular Biology, Vol.. 2037 https://doi.org/10.1007/978-1-4939-9690-2_21