Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
Original languageEnglish
Title of host publicationNMR-Based Metabolomics
EditorsG. Gowda, D. Raftery
PublisherSpringer
Publication date2019
Pages385-393
Chapter21
ISBN (Print)978-1-4939-9689-6
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume2037
ISSN1064-3745

Keywords

  • dDNP
  • Hyperpolarization
  • 13C-NMR
  • Glucose

Cite this

Lerche, M. H., Karlsson, M., Ardenkjær-Larsen, J. H., & Jensen, P. R. (2019). Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. In G. Gowda, & D. Raftery (Eds.), NMR-Based Metabolomics (pp. 385-393). Springer. Methods in Molecular Biology, Vol.. 2037 https://doi.org/10.1007/978-1-4939-9690-2_21
Lerche, Mathilde Hauge ; Karlsson, Magnus ; Ardenkjær-Larsen, Jan Henrik ; Jensen, Pernille Rose. / Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. NMR-Based Metabolomics. editor / G. Gowda ; D. Raftery . Springer, 2019. pp. 385-393 (Methods in Molecular Biology, Vol. 2037).
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Lerche, MH, Karlsson, M, Ardenkjær-Larsen, JH & Jensen, PR 2019, Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. in G Gowda & D Raftery (eds), NMR-Based Metabolomics. Springer, Methods in Molecular Biology, vol. 2037, pp. 385-393. https://doi.org/10.1007/978-1-4939-9690-2_21

Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. / Lerche, Mathilde Hauge; Karlsson, Magnus; Ardenkjær-Larsen, Jan Henrik; Jensen, Pernille Rose.

NMR-Based Metabolomics. ed. / G. Gowda; D. Raftery . Springer, 2019. p. 385-393 (Methods in Molecular Biology, Vol. 2037).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

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T1 - Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

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AU - Jensen, Pernille Rose

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AB - Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.

KW - dDNP

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Lerche MH, Karlsson M, Ardenkjær-Larsen JH, Jensen PR. Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. In Gowda G, Raftery D, editors, NMR-Based Metabolomics. Springer. 2019. p. 385-393. (Methods in Molecular Biology, Vol. 2037). https://doi.org/10.1007/978-1-4939-9690-2_21