@inbook{43d86d13ee5b4a809f3acb75370261bf,
title = "Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization",
abstract = "Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.",
keywords = "dDNP, Hyperpolarization, 13C-NMR, Glucose",
author = "Lerche, {Mathilde Hauge} and Magnus Karlsson and Ardenkj{\ae}r-Larsen, {Jan Henrik} and Jensen, {Pernille Rose}",
year = "2019",
doi = "10.1007/978-1-4939-9690-2_21",
language = "English",
isbn = "978-1-4939-9689-6",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "385--393",
editor = "G. Gowda and {Raftery }, D.",
booktitle = "NMR-Based Metabolomics",
}