Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

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Abstract

Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
Original languageEnglish
Title of host publicationNMR-Based Metabolomics
EditorsG. Gowda, D. Raftery
PublisherSpringer
Publication date2019
Pages385-393
Chapter21
ISBN (Print)978-1-4939-9689-6
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume2037
ISSN1064-3745

Keywords

  • dDNP
  • Hyperpolarization
  • 13C-NMR
  • Glucose

Cite this

Lerche, M. H., Karlsson, M., Ardenkjær-Larsen, J. H., & Jensen, P. R. (2019). Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. In G. Gowda, & D. Raftery (Eds.), NMR-Based Metabolomics (pp. 385-393). Springer. Methods in Molecular Biology, Vol.. 2037 https://doi.org/10.1007/978-1-4939-9690-2_21