Abstract
| Original language | English |
|---|---|
| Title of host publication | NMR-Based Metabolomics |
| Editors | G. Gowda, D. Raftery |
| Publisher | Springer |
| Publication date | 2019 |
| Pages | 385-393 |
| Chapter | 21 |
| ISBN (Print) | 978-1-4939-9689-6 |
| DOIs | |
| Publication status | Published - 2019 |
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2037 |
| ISSN | 1064-3745 |
Keywords
- dDNP
- Hyperpolarization
- 13C-NMR
- Glucose
Cite this
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Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. / Lerche, Mathilde Hauge; Karlsson, Magnus; Ardenkjær-Larsen, Jan Henrik; Jensen, Pernille Rose.
NMR-Based Metabolomics. ed. / G. Gowda; D. Raftery . Springer, 2019. p. 385-393 (Methods in Molecular Biology, Vol. 2037).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
TY - CHAP
T1 - Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization
AU - Lerche, Mathilde Hauge
AU - Karlsson, Magnus
AU - Ardenkjær-Larsen, Jan Henrik
AU - Jensen, Pernille Rose
PY - 2019
Y1 - 2019
N2 - Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
AB - Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
KW - dDNP
KW - Hyperpolarization
KW - 13C-NMR
KW - Glucose
U2 - 10.1007/978-1-4939-9690-2_21
DO - 10.1007/978-1-4939-9690-2_21
M3 - Book chapter
C2 - 31463856
SN - 978-1-4939-9689-6
T3 - Methods in Molecular Biology
SP - 385
EP - 393
BT - NMR-Based Metabolomics
A2 - Gowda, G.
A2 - Raftery , D.
PB - Springer
ER -