Targeted degradomics in protein terminomics and protease substrate discovery

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Targeted degradomics integrates positional information into mass spectrometry-based targeted proteomics workflows and thereby enables analysis of proteolytic cleavage events with unprecedented specificity and sensitivity. Rapid progress in establishment of protease-substrate relations provides extensive degradomics target lists that now can be tested with help of selected and parallel reaction monitoring (S/PRM) in complex biological systems, where proteases act in physiological environments. In this minireview, we describe the general principles of targeted degradomics, outline the generic experimental workflow of the methodology and highlight recent and future applications in protease research.
Original languageEnglish
JournalBiological Chemistry
Issue number1
Number of pages17
Publication statusPublished - 2017


  • Degradomics
  • Parallel reaction monitoring
  • Protease substrates
  • Selected reaction monitoring
  • Targeted proteomics.


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