Tailored biosynthesis of gibberellin plant hormones in yeast

Kanchana Rueksomtawin Kildegaard, Jonathan Asmund Arnesen, Belén Adiego Pérez, Daniela Rago, Mette Kristensen, Andreas Klitgaard, Esben H. Hansen, Jørgen Hansen, Irina Borodina*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The application of small amounts of natural plant growth hormones, such as gibberellins (GAs), can increase the productivity and quality of many vegetable and fruit crops. However, gibberellin growth hormones usage is limited by the high cost of their production, which is currently based on fermentation of a natural fungal producer Fusarium fujikuroi that produces a mix of several GAs. We explored the potential of the oleaginous yeast Yarrowia lipolytica to produce specific profiles of GAs. Firstly, the production of the GA-precursor ent-kaurenoic acid (KA) at 3.75 mg/L was achieved by expression of biosynthetic enzymes from the plant Arabidopsis thaliana and upregulation of the mevalonate (MVA) pathway. We then built a GA4-producing strain by extending the GA-biosynthetic pathway and upregulating the MVA-pathway further, resulting in 17.29 mg/L GA4. Additional expression of the F. fujikoroi GA-biosynthetic enzymes resulted in the production of GA7 (trace amounts) and GA3 (2.93 mg/L). Lastly, through protein engineering and the expression of additional KA-biosynthetic genes, we increased the GA3-production 4.4-fold resulting in 12.81 mg/L. The developed system presents a promising resource for the recombinant production of specific gibberellins, identifying bottlenecks in GA biosynthesis, and discovering new GA biosynthetic genes. Classification: Biological Sciences, Applied Biological Sciences.
Original languageEnglish
JournalMetabolic Engineering
Volume66
Pages (from-to)1-11
Number of pages11
ISSN1096-7176
DOIs
Publication statusPublished - 2021

Keywords

  • Gibberellins
  • Plant growth hormones
  • Isoprenoids
  • Oleaginous yeast
  • Yarrowia lipolytica

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