Systems Biology – A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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Systems Biology – A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria. / Liu, Jianming; Chan, Siu Hung Joshua; Chen, Jun; Solem, Christian; Jensen, Peter Ruhdal.

In: Frontiers in Microbiology, Vol. 10, 876, 2019.

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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@article{b77ec0d40cce467f8ec7bd76fbf11951,
title = "Systems Biology – A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria",
abstract = "Lactic Acid Bacteria (LAB) are extensively employed in the production of various fermented foods, due to their safe status, ability to affect texture and flavor and finally due to the beneficial effect they have on shelf-life. More recently, LAB have also gained interest as production hosts for various useful compounds, particularly compounds with sensitive applications, such as food ingredients and therapeutics. As for all industrial microorganisms, it is important to have a good understanding of the physiology and metabolism of LAB in order to fully exploit their potential, and for this purpose, many systems biology approaches are available. Systems metabolic engineering, an approach that combines optimization of metabolic enzymes/pathways at the systems level, synthetic biology as well as in silico model simulation, has been used to build microbial cell factories for production of biofuels, food ingredients and biochemicals. When developing LAB for use in foods, genetic engineering is in general not an accepted approach. An alternative is to screen mutant libraries for candidates with desirable traits using high-throughput screening technologies or to use adaptive laboratory evolution to select for mutants with special properties. In both cases, by using omics data and data-driven technologies to scrutinize these, it is possible to find the underlying cause for the desired attributes of such mutants. This review aims to describe how systems biology tools can be used for obtaining both engineered as well as non-engineered LAB with novel and desired properties.",
keywords = "Food fermentation, Metabolic engineering, Strain development, Control analysis, Screening and selection",
author = "Jianming Liu and Chan, {Siu Hung Joshua} and Jun Chen and Christian Solem and Jensen, {Peter Ruhdal}",
year = "2019",
doi = "10.3389/fmicb.2019.00876",
language = "English",
volume = "10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Systems Biology – A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria

AU - Liu, Jianming

AU - Chan, Siu Hung Joshua

AU - Chen, Jun

AU - Solem, Christian

AU - Jensen, Peter Ruhdal

PY - 2019

Y1 - 2019

N2 - Lactic Acid Bacteria (LAB) are extensively employed in the production of various fermented foods, due to their safe status, ability to affect texture and flavor and finally due to the beneficial effect they have on shelf-life. More recently, LAB have also gained interest as production hosts for various useful compounds, particularly compounds with sensitive applications, such as food ingredients and therapeutics. As for all industrial microorganisms, it is important to have a good understanding of the physiology and metabolism of LAB in order to fully exploit their potential, and for this purpose, many systems biology approaches are available. Systems metabolic engineering, an approach that combines optimization of metabolic enzymes/pathways at the systems level, synthetic biology as well as in silico model simulation, has been used to build microbial cell factories for production of biofuels, food ingredients and biochemicals. When developing LAB for use in foods, genetic engineering is in general not an accepted approach. An alternative is to screen mutant libraries for candidates with desirable traits using high-throughput screening technologies or to use adaptive laboratory evolution to select for mutants with special properties. In both cases, by using omics data and data-driven technologies to scrutinize these, it is possible to find the underlying cause for the desired attributes of such mutants. This review aims to describe how systems biology tools can be used for obtaining both engineered as well as non-engineered LAB with novel and desired properties.

AB - Lactic Acid Bacteria (LAB) are extensively employed in the production of various fermented foods, due to their safe status, ability to affect texture and flavor and finally due to the beneficial effect they have on shelf-life. More recently, LAB have also gained interest as production hosts for various useful compounds, particularly compounds with sensitive applications, such as food ingredients and therapeutics. As for all industrial microorganisms, it is important to have a good understanding of the physiology and metabolism of LAB in order to fully exploit their potential, and for this purpose, many systems biology approaches are available. Systems metabolic engineering, an approach that combines optimization of metabolic enzymes/pathways at the systems level, synthetic biology as well as in silico model simulation, has been used to build microbial cell factories for production of biofuels, food ingredients and biochemicals. When developing LAB for use in foods, genetic engineering is in general not an accepted approach. An alternative is to screen mutant libraries for candidates with desirable traits using high-throughput screening technologies or to use adaptive laboratory evolution to select for mutants with special properties. In both cases, by using omics data and data-driven technologies to scrutinize these, it is possible to find the underlying cause for the desired attributes of such mutants. This review aims to describe how systems biology tools can be used for obtaining both engineered as well as non-engineered LAB with novel and desired properties.

KW - Food fermentation

KW - Metabolic engineering

KW - Strain development

KW - Control analysis

KW - Screening and selection

U2 - 10.3389/fmicb.2019.00876

DO - 10.3389/fmicb.2019.00876

M3 - Journal article

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 876

ER -