Systems analysis of methylerythritol-phosphate pathway flux in E.coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

Mareike Bongers, Panagiotis K. Chrysanthopoulos, James B. Y. H. Behrendorff, Mark P. Hodson, Claudia E. Vickers, Lars K. Nielsen

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigment lycopene, a carotenoid with antioxidant properties, has been used as a reporter of isoprenoid pathway flux in metabolic engineering for over a decade. Lycopene production is known to vary between wild-type Escherichia coli hosts, but the reasons behind this variation have never been fully elucidated.
Original languageEnglish
Article number193
JournalMicrobial Cell Factories
Volume14
Number of pages16
ISSN1475-2859
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Isoprenoids
  • Lycopene
  • Methylerythritol phosphate pathway
  • RpoS
  • ROS
  • Escherichia coli
  • Oxidative stress

Cite this

Bongers, Mareike ; Chrysanthopoulos, Panagiotis K. ; Behrendorff, James B. Y. H. ; Hodson, Mark P. ; Vickers, Claudia E. ; Nielsen, Lars K. / Systems analysis of methylerythritol-phosphate pathway flux in E.coli : insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite. In: Microbial Cell Factories. 2015 ; Vol. 14.
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abstract = "High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigment lycopene, a carotenoid with antioxidant properties, has been used as a reporter of isoprenoid pathway flux in metabolic engineering for over a decade. Lycopene production is known to vary between wild-type Escherichia coli hosts, but the reasons behind this variation have never been fully elucidated.",
keywords = "Isoprenoids, Lycopene, Methylerythritol phosphate pathway, RpoS, ROS, Escherichia coli, Oxidative stress",
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Systems analysis of methylerythritol-phosphate pathway flux in E.coli : insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite. / Bongers, Mareike; Chrysanthopoulos, Panagiotis K.; Behrendorff, James B. Y. H.; Hodson, Mark P.; Vickers, Claudia E.; Nielsen, Lars K.

In: Microbial Cell Factories, Vol. 14, 193, 2015.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Systems analysis of methylerythritol-phosphate pathway flux in E.coli

T2 - insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

AU - Bongers, Mareike

AU - Chrysanthopoulos, Panagiotis K.

AU - Behrendorff, James B. Y. H.

AU - Hodson, Mark P.

AU - Vickers, Claudia E.

AU - Nielsen, Lars K.

PY - 2015

Y1 - 2015

N2 - High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigment lycopene, a carotenoid with antioxidant properties, has been used as a reporter of isoprenoid pathway flux in metabolic engineering for over a decade. Lycopene production is known to vary between wild-type Escherichia coli hosts, but the reasons behind this variation have never been fully elucidated.

AB - High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigment lycopene, a carotenoid with antioxidant properties, has been used as a reporter of isoprenoid pathway flux in metabolic engineering for over a decade. Lycopene production is known to vary between wild-type Escherichia coli hosts, but the reasons behind this variation have never been fully elucidated.

KW - Isoprenoids

KW - Lycopene

KW - Methylerythritol phosphate pathway

KW - RpoS

KW - ROS

KW - Escherichia coli

KW - Oxidative stress

U2 - 10.1186/s12934-015-0381-7

DO - 10.1186/s12934-015-0381-7

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JO - Microbial Cell Factories

JF - Microbial Cell Factories

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