System-level modeling and simulation of the cell culture microfluidic biochip ProCell

Wajid Hassan Minhass, Paul Pop, Jan Madsen, Mette Hemmingsen, Martin Dufva

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    Abstract

    Microfluidic biochips offer a promising alternative to a conventional biochemical laboratory. There are two technologies for the microfluidic biochips: droplet-based and flow-based. In this paper we are interested in flow-based microfluidic biochips, where the liquid flows continuously through pre-defined micro-channels using valves and pumps. We present an approach to the system-level modeling and simulation of a cell culture microfluidic biochip called ProCell, Programmable Cell Culture Chip. ProCell contains a cell culture chamber, which is envisioned to run 256 simultaneous experiments (viewed as a 16 × 16 matrix). We use an inverted fluorescence microscope to observe the experiments in real-time, allowing kinetic data analysis. We are able to automatically adjust the current experimental setup thus allowing, for the first time, conditional experiments. We propose a biochip architecture model and a comprehensive fault model that captures permanent faults occurring during chip operation. Using the proposed modeling and simulation framework, we perform an architectural level evaluation of two cell culture chamber implementations. A qualitative success metric is also proposed to evaluate chip performance in the presence of partial failures. Our results show that significant improvements in efficiency can be obtained using redundancy, providing improved chances to complete an experiment even in the presence of faults. This decreases the experiment repetition rate while increasing system productivity, saving time and reducing costs.
    Original languageEnglish
    Title of host publication2010 Symposium on Design Test Integration and Packaging of MEMS/MOEMS
    PublisherIEEE
    Publication date2010
    Pages91-98
    ISBN (Print)978-1-4244-6636-8
    Publication statusPublished - 2010
    Event2010 Symposium on Design Test Integration and Packaging of MEMS/MOEMS - Seville, Spain
    Duration: 5 May 20107 May 2010
    http://www.ieee.org/conferences_events/conferences/conferencedetails/index.html?Conf_ID=16860

    Conference

    Conference2010 Symposium on Design Test Integration and Packaging of MEMS/MOEMS
    CountrySpain
    CitySeville
    Period05/05/201007/05/2010
    Internet address

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