Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria

Guanjing Cai; Gefu Zhu; Mingdian Zhou; Nan Lv; Ruming Wang; Chunxing Li; Junjie Li; Xiaofang Pan

doi:10.1016/j.biortech.2021.125074

Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria

Guanjing Cai, Gefu Zhu^*, Mingdian Zhou, Nan Lv, Ruming Wang, Chunxing Li, Junjie Li, Xiaofang Pan

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.

Original language	English
Article number	125074
Journal	Bioresource Technology
Volume	332
Number of pages	10
ISSN	0960-8524
DOIs	https://doi.org/10.1016/j.biortech.2021.125074
Publication status	Published - 2021

Bibliographical note

Funding Information:
The authors would like to thank the National Natural Science Foundation of China (Contract Nos. 21876167 , 52070176 , 51678553 , 22006146 ), the National Key Research and Development Program of China (Contract No. 2018YFD0500202-4 ), the Project of the Natural Science Foundation of Fujian Province (Contract No. 2019J05161 ) and the Project of Key Laboratory of Energy Resource Utilization from Agriculture Residue, Ministry of Agriculture and Rural Affairs , P.R. China (Contract No. KLERUAR2020-01 ) for their financial support.

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

Anaerobic digestion
Anthraquinone-2-sulfonate
Butyrate degradation
Cysteine
Methane production

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Cai, G., Zhu, G., Zhou, M., Lv, N., Wang, R., Li, C., Li, J., & Pan, X. (2021). Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria. Bioresource Technology, 332, Article 125074. https://doi.org/10.1016/j.biortech.2021.125074

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title = "Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria",

abstract = "Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.",

keywords = "Anaerobic digestion, Anthraquinone-2-sulfonate, Butyrate degradation, Cysteine, Methane production",

author = "Guanjing Cai and Gefu Zhu and Mingdian Zhou and Nan Lv and Ruming Wang and Chunxing Li and Junjie Li and Xiaofang Pan",

note = "Funding Information: The authors would like to thank the National Natural Science Foundation of China (Contract Nos. 21876167 , 52070176 , 51678553 , 22006146 ), the National Key Research and Development Program of China (Contract No. 2018YFD0500202-4 ), the Project of the Natural Science Foundation of Fujian Province (Contract No. 2019J05161 ) and the Project of Key Laboratory of Energy Resource Utilization from Agriculture Residue, Ministry of Agriculture and Rural Affairs , P.R. China (Contract No. KLERUAR2020-01 ) for their financial support. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2021",

doi = "10.1016/j.biortech.2021.125074",

language = "English",

volume = "332",

journal = "Bioresource Technology",

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Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria. / Cai, Guanjing; Zhu, Gefu; Zhou, Mingdian et al.
In: Bioresource Technology, Vol. 332, 125074, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine

T2 - Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria

AU - Cai, Guanjing

AU - Zhu, Gefu

AU - Zhou, Mingdian

AU - Lv, Nan

AU - Wang, Ruming

AU - Li, Chunxing

AU - Li, Junjie

AU - Pan, Xiaofang

N1 - Funding Information: The authors would like to thank the National Natural Science Foundation of China (Contract Nos. 21876167 , 52070176 , 51678553 , 22006146 ), the National Key Research and Development Program of China (Contract No. 2018YFD0500202-4 ), the Project of the Natural Science Foundation of Fujian Province (Contract No. 2019J05161 ) and the Project of Key Laboratory of Energy Resource Utilization from Agriculture Residue, Ministry of Agriculture and Rural Affairs , P.R. China (Contract No. KLERUAR2020-01 ) for their financial support. Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021

Y1 - 2021

N2 - Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.

AB - Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.

KW - Anaerobic digestion

KW - Anthraquinone-2-sulfonate

KW - Butyrate degradation

KW - Cysteine

KW - Methane production

U2 - 10.1016/j.biortech.2021.125074

DO - 10.1016/j.biortech.2021.125074

M3 - Journal article

C2 - 33838452

AN - SCOPUS:85103737184

SN - 0960-8524

VL - 332

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 125074

ER -

Syntrophic butyrate-oxidizing methanogenesis promoted by anthraquinone-2-sulfonate and cysteine: Distinct tendencies towards the enrichment of methanogens and syntrophic fatty-acid oxidizing bacteria

Abstract

Bibliographical note

Keywords

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