Synthetic promoters generated based on genomic dna sequences

Henrik Toft Simonsen (Inventor), Christopher Workman (Inventor), Kyle Rothschild-Mancinelli (Inventor)

Research output: Patent

6 Downloads (Pure)

Abstract

The present disclosure relates to a method for generating a synthetic promoter and/or terminator, the method comprising: obtaining gene sequences, such as functional sequences and/or coding sequences, of genomes of multiple closely related species; identifying activity regulating features, by calculating a correlation coefficient between parameters of the statistical model and features scores of the FMS, and recognizing said activity regulating features as model features of which said correlation coefficient is above a predetermined threshold, such as 0.5, optimizing, for each HMM, the starting consensus sequence, by, for each activity regulating feature, calculating the gene statistics upon inserting/replacing the activity regulating feature into the starting consensus sequence or a partially optimized consensus sequence, and where said insertion/replacement leads to an improvement in the gene statistics, such as an increase by a predetermined threshold, accepting said insertion/replacement, otherwise said insertion/replacement is discarded.

Original languageEnglish
IPCG16B 40/ 20 A I
Patent numberWO2023135151
Filing date11/01/2022
Country/TerritoryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date11/01/2022
Priority numberEP20220150941
Publication statusPublished - 20 Jul 2023

Fingerprint

Dive into the research topics of 'Synthetic promoters generated based on genomic dna sequences'. Together they form a unique fingerprint.

Cite this