Abstract
The present disclosure relates to a method for generating a synthetic promoter and/or terminator, the method comprising: obtaining gene sequences, such as functional sequences and/or coding sequences, of genomes of multiple closely related species; identifying activity regulating features, by calculating a correlation coefficient between parameters of the statistical model and features scores of the FMS, and recognizing said activity regulating features as model features of which said correlation coefficient is above a predetermined threshold, such as 0.5, optimizing, for each HMM, the starting consensus sequence, by, for each activity regulating feature, calculating the gene statistics upon inserting/replacing the activity regulating feature into the starting consensus sequence or a partially optimized consensus sequence, and where said insertion/replacement leads to an improvement in the gene statistics, such as an increase by a predetermined threshold, accepting said insertion/replacement, otherwise said insertion/replacement is discarded.
| Original language | English |
|---|---|
| IPC | G16B 40/ 20 A I |
| Patent number | WO2023135151 |
| Filing date | 11/01/2022 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 11/01/2022 |
| Priority number | EP20220150941 |
| Publication status | Published - 20 Jul 2023 |