Synthetic multi-component enzyme mixtures for deconstruction of lignocellulosic biomass

Goutami Banerjee, Suzana Car, John S. Scott-Craig, Melissa S. Borrusch, Mareike Bongers, Jonathan D. Walton

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A high throughput enzyme assay platform, called GENPLAT, was used to guide the development of an optimized mixture of individual purified enzymes from ten "accessory" and six "core" enzymes. Enzyme mixtures were optimized for release of Glu, Xyl, or a combination of the two from corn stover pretreated by ammonia-fiber expansion (AFEX). Assay conditions were a fixed enzyme loading of 15 mg/g glucan, 48 h digestion, and 50 degrees C. Five of the ten tested accessory proteins enhanced Glu or Xyl yield compared to the core set alone, and five did not. An 11-component mixture containing the core set and five accessory enzymes optimized for Glu released 52.1% of the available Glu, compared to 38.5% with the core set alone. A mixture optimized for Xyl released 39.9% of the Xyl, compared to 26.4% with the core set alone. We predict that there is still considerable opportunity for further improvement of synthetic mixtures. Furthermore, the strategy described here is applicable to the development of more efficient enzyme cocktails for any pretreatment/biomass combination and for detecting enzymes that make a heretofore unrecognized contribution to lignocellulose deconstruction.
Original languageEnglish
JournalBioresource Technology
Volume101
Issue number23
Pages (from-to)9097-9105
Number of pages9
ISSN0960-8524
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Ammonia-fiber expansion
  • Cellulase
  • Lignocellulose
  • Pichia pastoris
  • Trichoderma reesei

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