Synthetic metabolism for biohalogenation

Antonin Cros; Gabriela Alfaro-Espinoza; Alberto De Maria; Nicolas T. Wirth; Pablo I. Nikel

doi:10.1016/j.copbio.2021.11.009

Synthetic metabolism for biohalogenation

Antonin Cros
, Gabriela Alfaro-Espinoza
, Alberto De Maria
, Nicolas T. Wirth
, Pablo I. Nikel

Max Planck Institute of Molecular Plant Physiology
Federal Institute for Materials Research and Testing Berlin

Research output: Contribution to journal › Review › peer-review

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Abstract

The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.

Original language	English
Journal	Current Opinion in Biotechnology
Volume	74
Pages (from-to)	180-193
Number of pages	14
ISSN	0958-1669
DOIs	https://doi.org/10.1016/j.copbio.2021.11.009
Publication status	Published - 2022

Bibliographical note

Funding Information:
We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580 , LiFe ( NNF18OC0034818 ) and TARGET ( NNF21OC0067996 ), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged.

Funding Information:
We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580, LiFe (NNF18OC0034818) and TARGET (NNF21OC0067996), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged.

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© 2021 The Authors

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title = "Synthetic metabolism for biohalogenation",

abstract = "The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.",

author = "Antonin Cros and Gabriela Alfaro-Espinoza and \{De Maria\}, Alberto and Wirth, \{Nicolas T.\} and Nikel, \{Pablo I.\}",

note = "Funding Information: We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580 , LiFe ( NNF18OC0034818 ) and TARGET ( NNF21OC0067996 ), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union{\textquoteright}s Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged. Funding Information: We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580, LiFe (NNF18OC0034818) and TARGET (NNF21OC0067996), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2022",

doi = "10.1016/j.copbio.2021.11.009",

language = "English",

volume = "74",

pages = "180--193",

journal = "Current Opinion in Biotechnology",

issn = "0958-1669",

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}

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T1 - Synthetic metabolism for biohalogenation

AU - Cros, Antonin

AU - Alfaro-Espinoza, Gabriela

AU - De Maria, Alberto

AU - Wirth, Nicolas T.

AU - Nikel, Pablo I.

N1 - Funding Information: We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580 , LiFe ( NNF18OC0034818 ) and TARGET ( NNF21OC0067996 ), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged. Funding Information: We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580, LiFe (NNF18OC0034818) and TARGET (NNF21OC0067996), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged. Publisher Copyright: © 2021 The Authors

PY - 2022

Y1 - 2022

N2 - The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.

AB - The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.

U2 - 10.1016/j.copbio.2021.11.009

DO - 10.1016/j.copbio.2021.11.009

M3 - Review

C2 - 34954625

AN - SCOPUS:85121637308

SN - 0958-1669

VL - 74

SP - 180

EP - 193

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

ER -

Synthetic metabolism for biohalogenation

Abstract

Bibliographical note

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