Abstract
The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.
| Original language | English |
|---|---|
| Journal | Current Opinion in Biotechnology |
| Volume | 74 |
| Pages (from-to) | 180-193 |
| Number of pages | 14 |
| ISSN | 0958-1669 |
| DOIs | |
| Publication status | Published - 2022 |
Bibliographical note
Funding Information:We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580 , LiFe ( NNF18OC0034818 ) and TARGET ( NNF21OC0067996 ), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged.
Funding Information:
We are indebted to all members of the EU SinFonia consortium (Synthetic biology-guided engineering of Pseudomonas putida for biofluorination) for fruitful exchanges and discussions. Also, we would like to acknowledge the work of the many researchers who made authoritative contributions to the field of halogenation and microbial organofluorine biodegradation, the work of whom could not always be cited in this article because of space constraints. This article is dedicated to the memory of the late Arren Bar-Even, the vision of whom guided growth-coupled selection schemes currently exploited for synthetic biohalogenation. The financial support from The Novo Nordisk Foundation through grants NNF20CC0035580, LiFe (NNF18OC0034818) and TARGET (NNF21OC0067996), the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B), and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) to P.I.N. is gratefully acknowledged.
Publisher Copyright:
© 2021 The Authors