Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies

Arindam Maity, Claudia Macaubas, Elizabeth Mellins, Kira Astakhova

Research output: Contribution to journalJournal articleCommunication

37 Downloads (Pure)

Abstract

Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human 2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus.
Original languageEnglish
JournalMolecules
Volume20
Issue number6
Pages (from-to)10253-10263
Number of pages11
ISSN1420-3049
DOIs
Publication statusPublished - 2015
Externally publishedYes

Bibliographical note

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Keywords

  • CuAAC click chemistry
  • Antiphospholipid syndrome
  • Antigens
  • β2-glycoprotein I
  • Phosphoethanolamine
  • Prothrombin

Fingerprint Dive into the research topics of 'Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies'. Together they form a unique fingerprint.

Cite this